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Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model

A derivative formula, DGBX, which is composed of three herbs (Radix astragali, Radix Angelicae sinensis, and Coptis chinensis Franch), is derived from a famous Chinese herbal formula, Danggui Buxue Tang (DBT) (Radix astragali and Radix Angelicae sinensis). We aimed to investigate the effects of DGBX...

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Autores principales: Zhou, Jingwei, Li, Xue, Deng, Peiying, Wei, Yi, Liu, Juan, Chen, Meng, Xu, Yamei, Zhang, Dongmei, Zhu, Lingqun, Lou, Lixia, Dong, Bin, Jin, Qiushuo, Chai, Limin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603747/
https://www.ncbi.nlm.nih.gov/pubmed/28951880
http://dx.doi.org/10.1155/2017/9786972
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author Zhou, Jingwei
Li, Xue
Deng, Peiying
Wei, Yi
Liu, Juan
Chen, Meng
Xu, Yamei
Zhang, Dongmei
Zhu, Lingqun
Lou, Lixia
Dong, Bin
Jin, Qiushuo
Chai, Limin
author_facet Zhou, Jingwei
Li, Xue
Deng, Peiying
Wei, Yi
Liu, Juan
Chen, Meng
Xu, Yamei
Zhang, Dongmei
Zhu, Lingqun
Lou, Lixia
Dong, Bin
Jin, Qiushuo
Chai, Limin
author_sort Zhou, Jingwei
collection PubMed
description A derivative formula, DGBX, which is composed of three herbs (Radix astragali, Radix Angelicae sinensis, and Coptis chinensis Franch), is derived from a famous Chinese herbal formula, Danggui Buxue Tang (DBT) (Radix astragali and Radix Angelicae sinensis). We aimed to investigate the effects of DGBX on the regulation of the balance between proliferation and apoptosis of hematopoietic stem cells (HSCs) due to the aberrant immune response in a mouse model of aplastic anemia (AA). Cyclosporine (CsA), an immunosuppressor, was used as the positive control. Our results indicated that DGBX could downregulate the production of IFNγ in bone marrow cells by interfering with the binding between SLAM and SAP and the expressions of Fyn and T-bet. This herbal formula can also inhibit the activation of Fas-mediated apoptosis, interferon regulatory factor-1-induced JAK/Stat, and eukaryotic initiation factor 2 signaling pathways and thereby induce proliferation and attenuate apoptosis of HSCs. In conclusion, DGBX can relieve the immune-mediated destruction of HSCs, repair hematopoietic failure, and recover the hematopoietic function of HSCs in hematogenesis. Therefore, DGBX can be used in traditional medicine against AA as a complementary and alternative immunosuppressive therapeutic formula.
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spelling pubmed-56037472017-09-26 Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model Zhou, Jingwei Li, Xue Deng, Peiying Wei, Yi Liu, Juan Chen, Meng Xu, Yamei Zhang, Dongmei Zhu, Lingqun Lou, Lixia Dong, Bin Jin, Qiushuo Chai, Limin J Immunol Res Research Article A derivative formula, DGBX, which is composed of three herbs (Radix astragali, Radix Angelicae sinensis, and Coptis chinensis Franch), is derived from a famous Chinese herbal formula, Danggui Buxue Tang (DBT) (Radix astragali and Radix Angelicae sinensis). We aimed to investigate the effects of DGBX on the regulation of the balance between proliferation and apoptosis of hematopoietic stem cells (HSCs) due to the aberrant immune response in a mouse model of aplastic anemia (AA). Cyclosporine (CsA), an immunosuppressor, was used as the positive control. Our results indicated that DGBX could downregulate the production of IFNγ in bone marrow cells by interfering with the binding between SLAM and SAP and the expressions of Fyn and T-bet. This herbal formula can also inhibit the activation of Fas-mediated apoptosis, interferon regulatory factor-1-induced JAK/Stat, and eukaryotic initiation factor 2 signaling pathways and thereby induce proliferation and attenuate apoptosis of HSCs. In conclusion, DGBX can relieve the immune-mediated destruction of HSCs, repair hematopoietic failure, and recover the hematopoietic function of HSCs in hematogenesis. Therefore, DGBX can be used in traditional medicine against AA as a complementary and alternative immunosuppressive therapeutic formula. Hindawi 2017 2017-08-16 /pmc/articles/PMC5603747/ /pubmed/28951880 http://dx.doi.org/10.1155/2017/9786972 Text en Copyright © 2017 Jingwei Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Jingwei
Li, Xue
Deng, Peiying
Wei, Yi
Liu, Juan
Chen, Meng
Xu, Yamei
Zhang, Dongmei
Zhu, Lingqun
Lou, Lixia
Dong, Bin
Jin, Qiushuo
Chai, Limin
Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title_full Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title_fullStr Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title_full_unstemmed Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title_short Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model
title_sort chinese herbal formula, modified danggui buxue tang, attenuates apoptosis of hematopoietic stem cells in immune-mediated aplastic anemia mouse model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603747/
https://www.ncbi.nlm.nih.gov/pubmed/28951880
http://dx.doi.org/10.1155/2017/9786972
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