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Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells

Long non-coding RNAs (lncRNAs) are non-protein coding transcripts that modulate mRNA and microRNA (miRNA) expression, thereby controlling multiple cellular processes, including transcriptional regulation of gene expression, cell differentiation and apoptosis. Ionizing radiation (IR), a strong cellul...

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Autores principales: Beer, Lucian, Nemec, Lucas, Wagner, Tanja, Ristl, Robin, Altenburger, Lukas M., Ankersmit, Hendrik Jan, Mildner, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603904/
https://www.ncbi.nlm.nih.gov/pubmed/27974506
http://dx.doi.org/10.1093/jrr/rrw111
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author Beer, Lucian
Nemec, Lucas
Wagner, Tanja
Ristl, Robin
Altenburger, Lukas M.
Ankersmit, Hendrik Jan
Mildner, Michael
author_facet Beer, Lucian
Nemec, Lucas
Wagner, Tanja
Ristl, Robin
Altenburger, Lukas M.
Ankersmit, Hendrik Jan
Mildner, Michael
author_sort Beer, Lucian
collection PubMed
description Long non-coding RNAs (lncRNAs) are non-protein coding transcripts that modulate mRNA and microRNA (miRNA) expression, thereby controlling multiple cellular processes, including transcriptional regulation of gene expression, cell differentiation and apoptosis. Ionizing radiation (IR), a strong cellular stressor, is known to influence gene expression of irradiated cells, mainly by activation of oxidative processes. Whether and how IR also affects lncRNA expression in human peripheral blood mononuclear cells (PBMCs) is still poorly understood. Exposure of PBMCs to IR dose-dependently activated p53 and its downstream target p21, ultimately leading to cell-cycle arrest and/or apoptosis. Cleavage of caspase-3, a specific process during apoptotic cell death, was detectable at doses as low as 30 Gy. Transcriptome analysis of 60 Gy–irradiated PBMCs revealed a strong time-dependent regulation of a variety of lncRNAs. Among many unknown lncRNAs we also identified a significant upregulation of Trp53cor1, MEG3 and TUG1, which have been shown to be involved in the regulation of cell cycle and apoptotic processes mediated by p53. In addition, we found 177 miRNAs regulated in the same samples, including several miRNAs that are known targets of upregulated lncRNAs. Our data show that IR dose-dependently regulates the expression of a wide spectrum of lncRNAs in PBMCs, suggesting a crucial role for lncRNAs in the complex regulatory machinery activated in response to IR.
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spelling pubmed-56039042017-09-25 Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells Beer, Lucian Nemec, Lucas Wagner, Tanja Ristl, Robin Altenburger, Lukas M. Ankersmit, Hendrik Jan Mildner, Michael J Radiat Res Biology Long non-coding RNAs (lncRNAs) are non-protein coding transcripts that modulate mRNA and microRNA (miRNA) expression, thereby controlling multiple cellular processes, including transcriptional regulation of gene expression, cell differentiation and apoptosis. Ionizing radiation (IR), a strong cellular stressor, is known to influence gene expression of irradiated cells, mainly by activation of oxidative processes. Whether and how IR also affects lncRNA expression in human peripheral blood mononuclear cells (PBMCs) is still poorly understood. Exposure of PBMCs to IR dose-dependently activated p53 and its downstream target p21, ultimately leading to cell-cycle arrest and/or apoptosis. Cleavage of caspase-3, a specific process during apoptotic cell death, was detectable at doses as low as 30 Gy. Transcriptome analysis of 60 Gy–irradiated PBMCs revealed a strong time-dependent regulation of a variety of lncRNAs. Among many unknown lncRNAs we also identified a significant upregulation of Trp53cor1, MEG3 and TUG1, which have been shown to be involved in the regulation of cell cycle and apoptotic processes mediated by p53. In addition, we found 177 miRNAs regulated in the same samples, including several miRNAs that are known targets of upregulated lncRNAs. Our data show that IR dose-dependently regulates the expression of a wide spectrum of lncRNAs in PBMCs, suggesting a crucial role for lncRNAs in the complex regulatory machinery activated in response to IR. Oxford University Press 2017-03 2016-12-14 /pmc/articles/PMC5603904/ /pubmed/27974506 http://dx.doi.org/10.1093/jrr/rrw111 Text en © The Author 2016. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Biology
Beer, Lucian
Nemec, Lucas
Wagner, Tanja
Ristl, Robin
Altenburger, Lukas M.
Ankersmit, Hendrik Jan
Mildner, Michael
Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title_full Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title_fullStr Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title_full_unstemmed Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title_short Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
title_sort ionizing radiation regulates long non-coding rnas in human peripheral blood mononuclear cells
topic Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603904/
https://www.ncbi.nlm.nih.gov/pubmed/27974506
http://dx.doi.org/10.1093/jrr/rrw111
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