Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi

Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Wanping, Chen, Runfa, Liu, Qingpei, He, Yi, He, Kun, Ding, Xiaoli, Kang, Lijing, Guo, Xiaoxiao, Xie, Nana, Zhou, Youxiang, Lu, Yuanyuan, Cox, Russell J., Molnár, István, Li, Mu, Shao, Yanchun, Chen, Fusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603960/
https://www.ncbi.nlm.nih.gov/pubmed/28959415
http://dx.doi.org/10.1039/c7sc00475c
Descripción
Sumario:Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general.

Ejemplares similares