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Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Royal Society of Chemistry
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603960/ https://www.ncbi.nlm.nih.gov/pubmed/28959415 http://dx.doi.org/10.1039/c7sc00475c |
| _version_ | 1783264793302401024 |
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| author | Chen, Wanping Chen, Runfa Liu, Qingpei He, Yi He, Kun Ding, Xiaoli Kang, Lijing Guo, Xiaoxiao Xie, Nana Zhou, Youxiang Lu, Yuanyuan Cox, Russell J. Molnár, István Li, Mu Shao, Yanchun Chen, Fusheng |
| author_facet | Chen, Wanping Chen, Runfa Liu, Qingpei He, Yi He, Kun Ding, Xiaoli Kang, Lijing Guo, Xiaoxiao Xie, Nana Zhou, Youxiang Lu, Yuanyuan Cox, Russell J. Molnár, István Li, Mu Shao, Yanchun Chen, Fusheng |
| author_sort | Chen, Wanping |
| collection | PubMed |
| description | Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general. |
| format | Online Article Text |
| id | pubmed-5603960 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-56039602017-09-28 Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi Chen, Wanping Chen, Runfa Liu, Qingpei He, Yi He, Kun Ding, Xiaoli Kang, Lijing Guo, Xiaoxiao Xie, Nana Zhou, Youxiang Lu, Yuanyuan Cox, Russell J. Molnár, István Li, Mu Shao, Yanchun Chen, Fusheng Chem Sci Chemistry Monascus azaphilone pigments (MonAzPs) are very widely used as food colorants, but their biosynthetic pathway has remained poorly characterized for more than half a century. In this study, the individual steps of MonAzPs biosynthesis in Monascus ruber M7 were elucidated by a combination of targeted gene knockouts, heterologous gene expression, and in vitro chemical and enzymatic reactions. This study describes the first rational engineering of MonAzPs biosynthesis and provides a roadmap for future pathway engineering efforts directed towards the selective production of the most valuable pigments and serves as a model for the biosynthesis of fungal azaphilones in general. Royal Society of Chemistry 2017-07-01 2017-04-24 /pmc/articles/PMC5603960/ /pubmed/28959415 http://dx.doi.org/10.1039/c7sc00475c Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Chemistry Chen, Wanping Chen, Runfa Liu, Qingpei He, Yi He, Kun Ding, Xiaoli Kang, Lijing Guo, Xiaoxiao Xie, Nana Zhou, Youxiang Lu, Yuanyuan Cox, Russell J. Molnár, István Li, Mu Shao, Yanchun Chen, Fusheng Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi |
| title | Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
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| title_full | Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
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| title_fullStr | Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
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| title_full_unstemmed | Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
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| title_short | Orange, red, yellow: biosynthesis of azaphilone pigments in Monascus fungi
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| title_sort | orange, red, yellow: biosynthesis of azaphilone pigments in monascus fungi |
| topic | Chemistry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603960/ https://www.ncbi.nlm.nih.gov/pubmed/28959415 http://dx.doi.org/10.1039/c7sc00475c |
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