Evaluation Analysis of miRNAs Overexpression in Liquid-Based Cytology Endometrial Samples

Background: miRNAs have an important role as their deregulation is linked to endometrial cancer. Methods: A custom miScript® miRNA PCR Array was used to investigate for the first time the expression of eight miRNAs in forty-nine histologically confirmed Liquid Based cytology endometrial samples. The...

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Detalles Bibliográficos
Autores principales: Kottaridi, Christine, Spathis, Aris, Margari, Niki, Koureas, Nikolaos, Terzakis, Emmanouil, Chrelias, Charalampos, Pappas, Asimakis, Bilirakis, Evripidis, Pouliakis, Abraham, Panayiotides, Ioannis J, Karakitsos, Petros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604201/
https://www.ncbi.nlm.nih.gov/pubmed/28928858
http://dx.doi.org/10.7150/jca.19947
Descripción
Sumario:Background: miRNAs have an important role as their deregulation is linked to endometrial cancer. Methods: A custom miScript® miRNA PCR Array was used to investigate for the first time the expression of eight miRNAs in forty-nine histologically confirmed Liquid Based cytology endometrial samples. The expression profile of the same miRNAs was also examined in sixty formalin-fixed tissue samples. Results: Expression of seven miRNAs was significantly higher in malignant samples with three of them (mir-182, mir-141 and mir-205) performing optimally. Conclusion: These results suggest the potential use of this non-invasive method of sampling for miRNA expression studies. Furthermore miRNA overexpression could serve as an ancillary or reflex test for optimal identification of malignant samples especially in morphologically inadequate samples.

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