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Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection
The emergence of multiple novel lineages of H1 and H3 influenza A viruses in swine has confounded control by inactivated vaccines. Because of substantial genetic and geographic heterogeneity among circulating swine influenza viruses, one vaccine strain per subtype cannot be efficacious against all o...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604745/ https://www.ncbi.nlm.nih.gov/pubmed/29250437 http://dx.doi.org/10.1038/s41541-017-0012-x |
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author | Van Reeth, Kristien Mancera Gracia, José Carlos Trus, Ivan Sys, Lieve Claes, Gerwin Versnaeyen, Han Cox, Eric Krammer, Florian Qiu, Yu |
author_facet | Van Reeth, Kristien Mancera Gracia, José Carlos Trus, Ivan Sys, Lieve Claes, Gerwin Versnaeyen, Han Cox, Eric Krammer, Florian Qiu, Yu |
author_sort | Van Reeth, Kristien |
collection | PubMed |
description | The emergence of multiple novel lineages of H1 and H3 influenza A viruses in swine has confounded control by inactivated vaccines. Because of substantial genetic and geographic heterogeneity among circulating swine influenza viruses, one vaccine strain per subtype cannot be efficacious against all of the current lineages. We have performed vaccination-challenge studies in pigs to examine whether priming and booster vaccinations with antigenically distinct H3N2 swine influenza viruses could broaden antibody responses and protection. We prepared monovalent whole inactivated, adjuvanted vaccines based on a European and a North American H3N2 swine influenza virus, which showed 81.5% aa homology in the HA1 region of the hemagglutinin and 83.4% in the neuraminidase. Our data show that (i) Priming with European and boosting with North American H3N2 swine influenza virus induces antibodies and protection against both vaccine strains, unlike prime-boost vaccination with a single virus or a single administration of bivalent vaccine. (ii) The heterologous prime-boost vaccination enhances hemagglutination inhibiting, virus neutralizing and neuraminidase inhibiting antibody responses against H3N2 viruses that are antigenically distinct from both vaccine strains. Antibody titers to the most divergent viruses were higher than after two administrations of bivalent vaccine. (iii) However, it does not induce antibodies to the conserved hemagglutinin stalk or to other hemagglutinin subtypes. We conclude that heterologous prime-boost vaccination might broaden protection to H3N2 swine influenza viruses and reduce the total amount of vaccine needed. This strategy holds potential for vaccination against influenza viruses from both humans and swine and for a better control of (reverse) zoonotic transmission of influenza viruses. |
format | Online Article Text |
id | pubmed-5604745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56047452017-12-14 Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection Van Reeth, Kristien Mancera Gracia, José Carlos Trus, Ivan Sys, Lieve Claes, Gerwin Versnaeyen, Han Cox, Eric Krammer, Florian Qiu, Yu NPJ Vaccines Article The emergence of multiple novel lineages of H1 and H3 influenza A viruses in swine has confounded control by inactivated vaccines. Because of substantial genetic and geographic heterogeneity among circulating swine influenza viruses, one vaccine strain per subtype cannot be efficacious against all of the current lineages. We have performed vaccination-challenge studies in pigs to examine whether priming and booster vaccinations with antigenically distinct H3N2 swine influenza viruses could broaden antibody responses and protection. We prepared monovalent whole inactivated, adjuvanted vaccines based on a European and a North American H3N2 swine influenza virus, which showed 81.5% aa homology in the HA1 region of the hemagglutinin and 83.4% in the neuraminidase. Our data show that (i) Priming with European and boosting with North American H3N2 swine influenza virus induces antibodies and protection against both vaccine strains, unlike prime-boost vaccination with a single virus or a single administration of bivalent vaccine. (ii) The heterologous prime-boost vaccination enhances hemagglutination inhibiting, virus neutralizing and neuraminidase inhibiting antibody responses against H3N2 viruses that are antigenically distinct from both vaccine strains. Antibody titers to the most divergent viruses were higher than after two administrations of bivalent vaccine. (iii) However, it does not induce antibodies to the conserved hemagglutinin stalk or to other hemagglutinin subtypes. We conclude that heterologous prime-boost vaccination might broaden protection to H3N2 swine influenza viruses and reduce the total amount of vaccine needed. This strategy holds potential for vaccination against influenza viruses from both humans and swine and for a better control of (reverse) zoonotic transmission of influenza viruses. Nature Publishing Group UK 2017-04-20 /pmc/articles/PMC5604745/ /pubmed/29250437 http://dx.doi.org/10.1038/s41541-017-0012-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Van Reeth, Kristien Mancera Gracia, José Carlos Trus, Ivan Sys, Lieve Claes, Gerwin Versnaeyen, Han Cox, Eric Krammer, Florian Qiu, Yu Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title | Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title_full | Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title_fullStr | Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title_full_unstemmed | Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title_short | Heterologous prime-boost vaccination with H3N2 influenza viruses of swine favors cross-clade antibody responses and protection |
title_sort | heterologous prime-boost vaccination with h3n2 influenza viruses of swine favors cross-clade antibody responses and protection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604745/ https://www.ncbi.nlm.nih.gov/pubmed/29250437 http://dx.doi.org/10.1038/s41541-017-0012-x |
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