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Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain

Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula. The causative agent, Rift Valley fever phlebovirus (RVFV), belongs to the genus Phlebovirus in the family Phenuiviridae and causes high rates of abortions in ruminants, and hemorrhagic fever, en...

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Autores principales: Ly, Hoai J., Lokugamage, Nandadeva, Nishiyama, Shoko, Ikegami, Tetsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604998/
https://www.ncbi.nlm.nih.gov/pubmed/28926632
http://dx.doi.org/10.1371/journal.pone.0185194
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author Ly, Hoai J.
Lokugamage, Nandadeva
Nishiyama, Shoko
Ikegami, Tetsuro
author_facet Ly, Hoai J.
Lokugamage, Nandadeva
Nishiyama, Shoko
Ikegami, Tetsuro
author_sort Ly, Hoai J.
collection PubMed
description Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula. The causative agent, Rift Valley fever phlebovirus (RVFV), belongs to the genus Phlebovirus in the family Phenuiviridae and causes high rates of abortions in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral maintenance by mosquito vectors has led to sporadic RVF outbreaks in ruminants and humans in endemic countries, and effective vaccination of animals and humans may minimize the impact of this disease. A live-attenuated MP-12 vaccine strain is one of the best characterized RVFV strains, and was conditionally approved as a veterinary vaccine in the U.S. Live-attenuated RVF vaccines including MP-12 strain may form reassortant strains with other bunyavirus species. This study thus aimed to characterize the occurrence of genetic reassortment between the MP-12 strain and bunyavirus species closely related to RVFV. The Arumowot virus (AMTV) and Gouleako goukovirus (GOLV), are transmitted by mosquitoes in Africa. The results of this study showed that GOLV does not form detectable reassortant strains with the MP-12 strain in co-infected C6/36 cells. The AMTV also did not form any reassortant strains with MP-12 strain in co-infected C6/36 cells, due to the incompatibility among N, L, and Gn/Gc proteins. A lack of reassortant formation could be due to a functional incompatibility of N and L proteins derived from heterologous species, and due to a lack of packaging via heterologous Gn/Gc proteins. The MP-12 strain did, however, randomly exchange L-, M-, and S-segments with a genetic variant strain, rMP12-GM50, in culture cells. The MP-12 strain is thus unlikely to form any reassortant strains with AMTV or GOLV in nature.
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spelling pubmed-56049982017-09-28 Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain Ly, Hoai J. Lokugamage, Nandadeva Nishiyama, Shoko Ikegami, Tetsuro PLoS One Research Article Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula. The causative agent, Rift Valley fever phlebovirus (RVFV), belongs to the genus Phlebovirus in the family Phenuiviridae and causes high rates of abortions in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral maintenance by mosquito vectors has led to sporadic RVF outbreaks in ruminants and humans in endemic countries, and effective vaccination of animals and humans may minimize the impact of this disease. A live-attenuated MP-12 vaccine strain is one of the best characterized RVFV strains, and was conditionally approved as a veterinary vaccine in the U.S. Live-attenuated RVF vaccines including MP-12 strain may form reassortant strains with other bunyavirus species. This study thus aimed to characterize the occurrence of genetic reassortment between the MP-12 strain and bunyavirus species closely related to RVFV. The Arumowot virus (AMTV) and Gouleako goukovirus (GOLV), are transmitted by mosquitoes in Africa. The results of this study showed that GOLV does not form detectable reassortant strains with the MP-12 strain in co-infected C6/36 cells. The AMTV also did not form any reassortant strains with MP-12 strain in co-infected C6/36 cells, due to the incompatibility among N, L, and Gn/Gc proteins. A lack of reassortant formation could be due to a functional incompatibility of N and L proteins derived from heterologous species, and due to a lack of packaging via heterologous Gn/Gc proteins. The MP-12 strain did, however, randomly exchange L-, M-, and S-segments with a genetic variant strain, rMP12-GM50, in culture cells. The MP-12 strain is thus unlikely to form any reassortant strains with AMTV or GOLV in nature. Public Library of Science 2017-09-19 /pmc/articles/PMC5604998/ /pubmed/28926632 http://dx.doi.org/10.1371/journal.pone.0185194 Text en © 2017 Ly et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ly, Hoai J.
Lokugamage, Nandadeva
Nishiyama, Shoko
Ikegami, Tetsuro
Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title_full Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title_fullStr Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title_full_unstemmed Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title_short Risk analysis of inter-species reassortment through a Rift Valley fever phlebovirus MP-12 vaccine strain
title_sort risk analysis of inter-species reassortment through a rift valley fever phlebovirus mp-12 vaccine strain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604998/
https://www.ncbi.nlm.nih.gov/pubmed/28926632
http://dx.doi.org/10.1371/journal.pone.0185194
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