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Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster

Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H(2)O(2) induced stress in D. m...

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Autores principales: Subramanian, Perumal, Kaliyamoorthy, Kanimozhi, Jayapalan, Jaime Jacqueline, Abdul-Rahman, Puteri Shafinaz, Haji Hashim, Onn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605229/
https://www.ncbi.nlm.nih.gov/pubmed/28931163
http://dx.doi.org/10.1093/jisesa/iex040
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author Subramanian, Perumal
Kaliyamoorthy, Kanimozhi
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Haji Hashim, Onn
author_facet Subramanian, Perumal
Kaliyamoorthy, Kanimozhi
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Haji Hashim, Onn
author_sort Subramanian, Perumal
collection PubMed
description Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H(2)O(2) induced stress in D. melanogaster was investigated. D. melanogaster flies were divided into four groups (group I – control, group II – H(2)O(2) (acute exposure), group III – quercetin, and group IV – quercetin + H(2)O(2) treated). Negative geotaxis assay, oxidative stress indicators (protein carbonyls, thiobarbituric reactive substances [TBARS]), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione-S-transferase [GST], glutathione peroxidase, and reduced glutathione [GSH]) were measured at 4 h intervals over 24 h and temporal expression of heat shock protein-70 (Hsp70), Upd1 (homolog of IL-6 in Drosophila), and nitric oxide synthase (Nos) was analyzed by Western blotting. Groups II and IV showed altered biochemical rhythms (compared with controls). Decreased mesor values of negative geotaxis, SOD, CAT, GST, and GSH were noticed in H(2)O(2), increased mesor of oxidative stress indicators (TBARS and protein carbonyl content) and a reversibility of the rhythmic characteristics were conspicuous after quercetin treatment. The expression levels of Hsp70, Upd1, and Nos were noticeably maximum at 04:00. Significant elevation of expression by H(2)O(2) was nearly normalized by quercetin treatment. The possible mechanism by which quercetin modulates oxidant–antioxidant imbalance under oxidative stress could be ascribed to the modulation of the rhythmic properties. Our results will be helpful to understand the molecular interlink between circadian rhythm and oxidative stress mechanism.
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spelling pubmed-56052292017-09-27 Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster Subramanian, Perumal Kaliyamoorthy, Kanimozhi Jayapalan, Jaime Jacqueline Abdul-Rahman, Puteri Shafinaz Haji Hashim, Onn J Insect Sci Research Article Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H(2)O(2) induced stress in D. melanogaster was investigated. D. melanogaster flies were divided into four groups (group I – control, group II – H(2)O(2) (acute exposure), group III – quercetin, and group IV – quercetin + H(2)O(2) treated). Negative geotaxis assay, oxidative stress indicators (protein carbonyls, thiobarbituric reactive substances [TBARS]), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione-S-transferase [GST], glutathione peroxidase, and reduced glutathione [GSH]) were measured at 4 h intervals over 24 h and temporal expression of heat shock protein-70 (Hsp70), Upd1 (homolog of IL-6 in Drosophila), and nitric oxide synthase (Nos) was analyzed by Western blotting. Groups II and IV showed altered biochemical rhythms (compared with controls). Decreased mesor values of negative geotaxis, SOD, CAT, GST, and GSH were noticed in H(2)O(2), increased mesor of oxidative stress indicators (TBARS and protein carbonyl content) and a reversibility of the rhythmic characteristics were conspicuous after quercetin treatment. The expression levels of Hsp70, Upd1, and Nos were noticeably maximum at 04:00. Significant elevation of expression by H(2)O(2) was nearly normalized by quercetin treatment. The possible mechanism by which quercetin modulates oxidant–antioxidant imbalance under oxidative stress could be ascribed to the modulation of the rhythmic properties. Our results will be helpful to understand the molecular interlink between circadian rhythm and oxidative stress mechanism. Oxford University Press 2017-04-28 /pmc/articles/PMC5605229/ /pubmed/28931163 http://dx.doi.org/10.1093/jisesa/iex040 Text en © The Author 2017. Published by Oxford University Press on behalf of the Entomological Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Subramanian, Perumal
Kaliyamoorthy, Kanimozhi
Jayapalan, Jaime Jacqueline
Abdul-Rahman, Puteri Shafinaz
Haji Hashim, Onn
Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title_full Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title_fullStr Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title_full_unstemmed Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title_short Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster
title_sort influence of quercetin in the temporal regulation of redox homeostasis in drosophila melanogaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605229/
https://www.ncbi.nlm.nih.gov/pubmed/28931163
http://dx.doi.org/10.1093/jisesa/iex040
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