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Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs
AIM: To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis. METHODS: Twenty dogs aged 2-8 years were divided in research (aged 2 years, induction of osteoarthrosis - IOA) and intact group. Intact...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605353/ https://www.ncbi.nlm.nih.gov/pubmed/28979851 http://dx.doi.org/10.5312/wjo.v8.i9.681 |
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author | Stupina, Tatyana Shchudlo, Michael Stepanov, Michael |
author_facet | Stupina, Tatyana Shchudlo, Michael Stepanov, Michael |
author_sort | Stupina, Tatyana |
collection | PubMed |
description | AIM: To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis. METHODS: Twenty dogs aged 2-8 years were divided in research (aged 2 years, induction of osteoarthrosis - IOA) and intact group. Intact group included three subgroups (aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations (weight %) were investigated. RESULTS: X-ray electron probe microanalysis revealed non-uniform sulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus content. CONCLUSION: The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis. |
format | Online Article Text |
id | pubmed-5605353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-56053532017-10-04 Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs Stupina, Tatyana Shchudlo, Michael Stepanov, Michael World J Orthop Basic Study AIM: To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis. METHODS: Twenty dogs aged 2-8 years were divided in research (aged 2 years, induction of osteoarthrosis - IOA) and intact group. Intact group included three subgroups (aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations (weight %) were investigated. RESULTS: X-ray electron probe microanalysis revealed non-uniform sulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus content. CONCLUSION: The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis. Baishideng Publishing Group Inc 2017-09-18 /pmc/articles/PMC5605353/ /pubmed/28979851 http://dx.doi.org/10.5312/wjo.v8.i9.681 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
spellingShingle | Basic Study Stupina, Tatyana Shchudlo, Michael Stepanov, Michael Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title | Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title_full | Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title_fullStr | Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title_full_unstemmed | Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title_short | Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
title_sort | electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605353/ https://www.ncbi.nlm.nih.gov/pubmed/28979851 http://dx.doi.org/10.5312/wjo.v8.i9.681 |
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