Cargando…

Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice

At least 15 cases of human beings infected with H5N6 have been reported since 2014, of which at least nine were fatal. The highly pathogenic avian H5N6 influenza virus may pose a serious threat to both public health and the poultry industry. However, the molecular features promoting the adaptation o...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Chunmao, Zhao, Zongzheng, Guo, Zhendong, Zhang, Jiajie, Li, Jiaming, Yang, Yifei, Lu, Shaoxia, Wang, Zhongyi, Zhi, Min, Fu, Yingying, Yang, Xiaoyu, Liu, Lina, Zhang, Yi, Hua, Yuping, Liu, Linna, Chai, Hongliang, Qian, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605651/
https://www.ncbi.nlm.nih.gov/pubmed/28966609
http://dx.doi.org/10.3389/fmicb.2017.01763
_version_ 1783265023995412480
author Zhang, Chunmao
Zhao, Zongzheng
Guo, Zhendong
Zhang, Jiajie
Li, Jiaming
Yang, Yifei
Lu, Shaoxia
Wang, Zhongyi
Zhi, Min
Fu, Yingying
Yang, Xiaoyu
Liu, Lina
Zhang, Yi
Hua, Yuping
Liu, Linna
Chai, Hongliang
Qian, Jun
author_facet Zhang, Chunmao
Zhao, Zongzheng
Guo, Zhendong
Zhang, Jiajie
Li, Jiaming
Yang, Yifei
Lu, Shaoxia
Wang, Zhongyi
Zhi, Min
Fu, Yingying
Yang, Xiaoyu
Liu, Lina
Zhang, Yi
Hua, Yuping
Liu, Linna
Chai, Hongliang
Qian, Jun
author_sort Zhang, Chunmao
collection PubMed
description At least 15 cases of human beings infected with H5N6 have been reported since 2014, of which at least nine were fatal. The highly pathogenic avian H5N6 influenza virus may pose a serious threat to both public health and the poultry industry. However, the molecular features promoting the adaptation of avian H5N6 influenza viruses to mammalian hosts is not well understood. Here, we sequentially passaged an avian H5N6 influenza A virus (A/Northern Shoveler/Ningxia/488-53/2015) 10 times in mice to identify the adaptive amino acid substitutions that confer enhanced virulence to H5N6 in mammals. The 1st and 10th passages of the mouse-adapted H5N6 viruses were named P1 and P10, respectively. P1 and P10 displayed higher pathogenicity in mice than their parent strain. P10 showed significantly higher replication capability in vivo and could be detected in the brains of mice, whereas P1 displayed higher replication efficiency in their lungs but was not detectable in the brain. Similar to its parent strain, P10 remained no transmissible between guinea pigs. Using genome sequencing and alignment, multiple amino acid substitutions, including PB2 E627K, PB2 T23I, PA T97I, and HA R239H, were found in the adaptation of H5N6 to mice. In summary, we identified amino acid changes that are associated with H5N6 adaptation to mice.
format Online
Article
Text
id pubmed-5605651
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-56056512017-09-29 Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice Zhang, Chunmao Zhao, Zongzheng Guo, Zhendong Zhang, Jiajie Li, Jiaming Yang, Yifei Lu, Shaoxia Wang, Zhongyi Zhi, Min Fu, Yingying Yang, Xiaoyu Liu, Lina Zhang, Yi Hua, Yuping Liu, Linna Chai, Hongliang Qian, Jun Front Microbiol Microbiology At least 15 cases of human beings infected with H5N6 have been reported since 2014, of which at least nine were fatal. The highly pathogenic avian H5N6 influenza virus may pose a serious threat to both public health and the poultry industry. However, the molecular features promoting the adaptation of avian H5N6 influenza viruses to mammalian hosts is not well understood. Here, we sequentially passaged an avian H5N6 influenza A virus (A/Northern Shoveler/Ningxia/488-53/2015) 10 times in mice to identify the adaptive amino acid substitutions that confer enhanced virulence to H5N6 in mammals. The 1st and 10th passages of the mouse-adapted H5N6 viruses were named P1 and P10, respectively. P1 and P10 displayed higher pathogenicity in mice than their parent strain. P10 showed significantly higher replication capability in vivo and could be detected in the brains of mice, whereas P1 displayed higher replication efficiency in their lungs but was not detectable in the brain. Similar to its parent strain, P10 remained no transmissible between guinea pigs. Using genome sequencing and alignment, multiple amino acid substitutions, including PB2 E627K, PB2 T23I, PA T97I, and HA R239H, were found in the adaptation of H5N6 to mice. In summary, we identified amino acid changes that are associated with H5N6 adaptation to mice. Frontiers Media S.A. 2017-09-15 /pmc/articles/PMC5605651/ /pubmed/28966609 http://dx.doi.org/10.3389/fmicb.2017.01763 Text en Copyright © 2017 Zhang, Zhao, Guo, Zhang, Li, Yang, Lu, Wang, Zhi, Fu, Yang, Liu, Zhang, Hua, Liu, Chai and Qian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Chunmao
Zhao, Zongzheng
Guo, Zhendong
Zhang, Jiajie
Li, Jiaming
Yang, Yifei
Lu, Shaoxia
Wang, Zhongyi
Zhi, Min
Fu, Yingying
Yang, Xiaoyu
Liu, Lina
Zhang, Yi
Hua, Yuping
Liu, Linna
Chai, Hongliang
Qian, Jun
Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title_full Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title_fullStr Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title_full_unstemmed Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title_short Amino Acid Substitutions Associated with Avian H5N6 Influenza A Virus Adaptation to Mice
title_sort amino acid substitutions associated with avian h5n6 influenza a virus adaptation to mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605651/
https://www.ncbi.nlm.nih.gov/pubmed/28966609
http://dx.doi.org/10.3389/fmicb.2017.01763
work_keys_str_mv AT zhangchunmao aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT zhaozongzheng aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT guozhendong aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT zhangjiajie aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT lijiaming aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT yangyifei aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT lushaoxia aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT wangzhongyi aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT zhimin aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT fuyingying aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT yangxiaoyu aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT liulina aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT zhangyi aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT huayuping aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT liulinna aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT chaihongliang aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice
AT qianjun aminoacidsubstitutionsassociatedwithavianh5n6influenzaavirusadaptationtomice