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Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices
Epilepsy and spreading depolarization (SD) are both episodic brain disorders and often exist together in the same individual. In CA1 pyramidal neurons of mouse hippocampal slices, induction of SD evoked epileptiform activities, including the ictal-like bursts, which occurred during the repolarizing...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605655/ https://www.ncbi.nlm.nih.gov/pubmed/28928441 http://dx.doi.org/10.1038/s41598-017-12346-y |
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author | Lin, Ching-Huei Hsu, Shih-Pin Cheng, Ting-Chun Huang, Chin-Wei Chiang, Yao-Chang Hsiao, I-Han Lee, Ming-Hsueh Shen, Mei-Lin Wu, Dong Chuan Zhou, Ning |
author_facet | Lin, Ching-Huei Hsu, Shih-Pin Cheng, Ting-Chun Huang, Chin-Wei Chiang, Yao-Chang Hsiao, I-Han Lee, Ming-Hsueh Shen, Mei-Lin Wu, Dong Chuan Zhou, Ning |
author_sort | Lin, Ching-Huei |
collection | PubMed |
description | Epilepsy and spreading depolarization (SD) are both episodic brain disorders and often exist together in the same individual. In CA1 pyramidal neurons of mouse hippocampal slices, induction of SD evoked epileptiform activities, including the ictal-like bursts, which occurred during the repolarizing phase of SD, and the subsequent generation of paroxysmal depolarization shifts (PDSs), which are characterized by mild depolarization plateau with overriding spikes. The duration of the ictal-like activity was correlated with both the recovery time and the depolarization potential of SD, whereas the parameters of PDSs were not significantly correlated with the parameters of SD. Moreover, we systematically evaluated the effects of multiple anti-epileptic drugs (AEDs) on SD-induced epileptiform activity. Among the drugs that are known to inhibit voltage-gated sodium channels, carbamazepine, phenytoin, valproate, lamotrigine, and zonisamide reduced the frequency of PDSs and the overriding firing bursts in 20–25 min after the induction of SD. The GABA uptake inhibitor tiagabine exhibited moderate effects and partially limited the incidence of PDSs after SD. AEDs including gabapentin, levetiracetam, ethosuximide, felbamate, and vigabatrin, had no significant effect on SD-induced epileptic activity. Taken together, these results demonstrate the effects of AEDs on SD and the related epileptiform activity at the cellular level. |
format | Online Article Text |
id | pubmed-5605655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56056552017-09-20 Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices Lin, Ching-Huei Hsu, Shih-Pin Cheng, Ting-Chun Huang, Chin-Wei Chiang, Yao-Chang Hsiao, I-Han Lee, Ming-Hsueh Shen, Mei-Lin Wu, Dong Chuan Zhou, Ning Sci Rep Article Epilepsy and spreading depolarization (SD) are both episodic brain disorders and often exist together in the same individual. In CA1 pyramidal neurons of mouse hippocampal slices, induction of SD evoked epileptiform activities, including the ictal-like bursts, which occurred during the repolarizing phase of SD, and the subsequent generation of paroxysmal depolarization shifts (PDSs), which are characterized by mild depolarization plateau with overriding spikes. The duration of the ictal-like activity was correlated with both the recovery time and the depolarization potential of SD, whereas the parameters of PDSs were not significantly correlated with the parameters of SD. Moreover, we systematically evaluated the effects of multiple anti-epileptic drugs (AEDs) on SD-induced epileptiform activity. Among the drugs that are known to inhibit voltage-gated sodium channels, carbamazepine, phenytoin, valproate, lamotrigine, and zonisamide reduced the frequency of PDSs and the overriding firing bursts in 20–25 min after the induction of SD. The GABA uptake inhibitor tiagabine exhibited moderate effects and partially limited the incidence of PDSs after SD. AEDs including gabapentin, levetiracetam, ethosuximide, felbamate, and vigabatrin, had no significant effect on SD-induced epileptic activity. Taken together, these results demonstrate the effects of AEDs on SD and the related epileptiform activity at the cellular level. Nature Publishing Group UK 2017-09-19 /pmc/articles/PMC5605655/ /pubmed/28928441 http://dx.doi.org/10.1038/s41598-017-12346-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lin, Ching-Huei Hsu, Shih-Pin Cheng, Ting-Chun Huang, Chin-Wei Chiang, Yao-Chang Hsiao, I-Han Lee, Ming-Hsueh Shen, Mei-Lin Wu, Dong Chuan Zhou, Ning Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title | Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title_full | Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title_fullStr | Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title_full_unstemmed | Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title_short | Effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
title_sort | effects of anti-epileptic drugs on spreading depolarization-induced epileptiform activity in mouse hippocampal slices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605655/ https://www.ncbi.nlm.nih.gov/pubmed/28928441 http://dx.doi.org/10.1038/s41598-017-12346-y |
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