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Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study

Surface colonization by microorganisms leads to the formation of biofilms, i.e. aggregates of bacteria embedded within a matrix of extracellular polymeric substance. This promotes adhesion to the surface and protects bacterial community, providing an antimicrobial-resistant environment. The inhibiti...

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Autores principales: Sportelli, M. C., Tütüncü, E., Picca, R. A., Valentini, M., Valentini, A., Kranz, C., Mizaikoff, B., Barth, H., Cioffi, N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605679/
https://www.ncbi.nlm.nih.gov/pubmed/28928400
http://dx.doi.org/10.1038/s41598-017-12088-x
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author Sportelli, M. C.
Tütüncü, E.
Picca, R. A.
Valentini, M.
Valentini, A.
Kranz, C.
Mizaikoff, B.
Barth, H.
Cioffi, N.
author_facet Sportelli, M. C.
Tütüncü, E.
Picca, R. A.
Valentini, M.
Valentini, A.
Kranz, C.
Mizaikoff, B.
Barth, H.
Cioffi, N.
author_sort Sportelli, M. C.
collection PubMed
description Surface colonization by microorganisms leads to the formation of biofilms, i.e. aggregates of bacteria embedded within a matrix of extracellular polymeric substance. This promotes adhesion to the surface and protects bacterial community, providing an antimicrobial-resistant environment. The inhibition of biofilm growth is a crucial issue for preventing bacterial infections. Inorganic nanoparticle/Teflon-like (CF(x)) composites deposited via ion beam sputtering demonstrated very efficient antimicrobial activity. In this study, we developed Ag-CF(x) thin films with tuneable metal loadings and exceptional in-plane morphological and chemical homogeneity. Ag-CF(x) antimicrobial activity was studied via mid-infrared attenuated total reflection spectroscopy utilizing specifically adapted multi-reflection waveguides. Biofilm was sampled by carefully depositing the Ag-CF(x) film on IR inactive regions of the waveguide. Real-time infrared spectroscopy was used to monitor Pseudomonas fluorescens biofilm growth inhibition induced by the bioactive silver ions released from the nanoantimicrobial coating. Few hours of Ag-CF(x) action were sufficient to affect significantly biofilm growth. These findings were corroborated by atomic force microscopy (AFM) studies on living bacteria exposed to the same nanoantimicrobial. Morphological analyses showed a severe bacterial stress, leading to membrane leakage/collapse or to extended cell lysis as a function of incubation time.
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spelling pubmed-56056792017-09-20 Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study Sportelli, M. C. Tütüncü, E. Picca, R. A. Valentini, M. Valentini, A. Kranz, C. Mizaikoff, B. Barth, H. Cioffi, N. Sci Rep Article Surface colonization by microorganisms leads to the formation of biofilms, i.e. aggregates of bacteria embedded within a matrix of extracellular polymeric substance. This promotes adhesion to the surface and protects bacterial community, providing an antimicrobial-resistant environment. The inhibition of biofilm growth is a crucial issue for preventing bacterial infections. Inorganic nanoparticle/Teflon-like (CF(x)) composites deposited via ion beam sputtering demonstrated very efficient antimicrobial activity. In this study, we developed Ag-CF(x) thin films with tuneable metal loadings and exceptional in-plane morphological and chemical homogeneity. Ag-CF(x) antimicrobial activity was studied via mid-infrared attenuated total reflection spectroscopy utilizing specifically adapted multi-reflection waveguides. Biofilm was sampled by carefully depositing the Ag-CF(x) film on IR inactive regions of the waveguide. Real-time infrared spectroscopy was used to monitor Pseudomonas fluorescens biofilm growth inhibition induced by the bioactive silver ions released from the nanoantimicrobial coating. Few hours of Ag-CF(x) action were sufficient to affect significantly biofilm growth. These findings were corroborated by atomic force microscopy (AFM) studies on living bacteria exposed to the same nanoantimicrobial. Morphological analyses showed a severe bacterial stress, leading to membrane leakage/collapse or to extended cell lysis as a function of incubation time. Nature Publishing Group UK 2017-09-19 /pmc/articles/PMC5605679/ /pubmed/28928400 http://dx.doi.org/10.1038/s41598-017-12088-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sportelli, M. C.
Tütüncü, E.
Picca, R. A.
Valentini, M.
Valentini, A.
Kranz, C.
Mizaikoff, B.
Barth, H.
Cioffi, N.
Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title_full Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title_fullStr Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title_full_unstemmed Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title_short Inhibiting P. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
title_sort inhibiting p. fluorescens biofilms with fluoropolymer-embedded silver nanoparticles: an in-situ spectroscopic study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605679/
https://www.ncbi.nlm.nih.gov/pubmed/28928400
http://dx.doi.org/10.1038/s41598-017-12088-x
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