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A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations

Glimepiride is one of the most widely prescribed antidiabetic drugs and contains both hydrophobic and hydrophilic functional groups in its molecules, and thus could be analyzed by either reversed-phase high performance liquid chromatography (HPLC) or hydrophilic interaction liquid chromatography (HI...

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Autores principales: Zhou, Si, Zuo, Pengxiao, Zuo, Yuegang, Deng, Yiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605893/
https://www.ncbi.nlm.nih.gov/pubmed/28951669
http://dx.doi.org/10.1016/j.jsps.2017.01.002
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author Zhou, Si
Zuo, Pengxiao
Zuo, Yuegang
Deng, Yiwei
author_facet Zhou, Si
Zuo, Pengxiao
Zuo, Yuegang
Deng, Yiwei
author_sort Zhou, Si
collection PubMed
description Glimepiride is one of the most widely prescribed antidiabetic drugs and contains both hydrophobic and hydrophilic functional groups in its molecules, and thus could be analyzed by either reversed-phase high performance liquid chromatography (HPLC) or hydrophilic interaction liquid chromatography (HILIC). In the literature, however, only reversed-phase HPLC has been reported. In this study, a simple, rapid and accurate hydrophilic interaction liquid chromatographic method was developed for the determination of glimepiride in pharmaceutical formulations. The analytical method comprised a fast ultrasound-assisted extraction with acetonitrile as a solvent followed by HILIC separation and quantification using a Waters Spherisorb S(5)NH(2) hydrophilic column with a mobile phase consisting of acetonitrile and aqueous acetate buffer (5.0 mM). The retention time of glimepiride increased slightly with decrease of mobile phase pH value from 6.8 to 5.8 and of acetonitrile content from 60% to 40%, indicating that both hydrophilic, ionic, and hydrophobic interactions were involved in the HILIC retention and elution mechanisms. Quantitation was carried out with a mobile phase of 40% acetonitrile and 60% aqueous acetate buffer (5.0 mM) at pH 6.3, by relating the peak area of glimepiride to that of the internal standard, with a detection limit of 15.0 μg/L. UV light absorption responses at 228 nm were linear over a wide concentration range from 50.0 μg/L to 6.00 mg/L. The recoveries of the standard added to pharmaceutical tablet samples were 99.4–103.0% for glimepiride, and the relative standard deviation for the analyte was less than 1.0%. This method has been successfully applied to determine the glimepiride contents in pharmaceutical formulations.
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spelling pubmed-56058932017-09-26 A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations Zhou, Si Zuo, Pengxiao Zuo, Yuegang Deng, Yiwei Saudi Pharm J Original Article Glimepiride is one of the most widely prescribed antidiabetic drugs and contains both hydrophobic and hydrophilic functional groups in its molecules, and thus could be analyzed by either reversed-phase high performance liquid chromatography (HPLC) or hydrophilic interaction liquid chromatography (HILIC). In the literature, however, only reversed-phase HPLC has been reported. In this study, a simple, rapid and accurate hydrophilic interaction liquid chromatographic method was developed for the determination of glimepiride in pharmaceutical formulations. The analytical method comprised a fast ultrasound-assisted extraction with acetonitrile as a solvent followed by HILIC separation and quantification using a Waters Spherisorb S(5)NH(2) hydrophilic column with a mobile phase consisting of acetonitrile and aqueous acetate buffer (5.0 mM). The retention time of glimepiride increased slightly with decrease of mobile phase pH value from 6.8 to 5.8 and of acetonitrile content from 60% to 40%, indicating that both hydrophilic, ionic, and hydrophobic interactions were involved in the HILIC retention and elution mechanisms. Quantitation was carried out with a mobile phase of 40% acetonitrile and 60% aqueous acetate buffer (5.0 mM) at pH 6.3, by relating the peak area of glimepiride to that of the internal standard, with a detection limit of 15.0 μg/L. UV light absorption responses at 228 nm were linear over a wide concentration range from 50.0 μg/L to 6.00 mg/L. The recoveries of the standard added to pharmaceutical tablet samples were 99.4–103.0% for glimepiride, and the relative standard deviation for the analyte was less than 1.0%. This method has been successfully applied to determine the glimepiride contents in pharmaceutical formulations. Elsevier 2017-09 2017-01-25 /pmc/articles/PMC5605893/ /pubmed/28951669 http://dx.doi.org/10.1016/j.jsps.2017.01.002 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zhou, Si
Zuo, Pengxiao
Zuo, Yuegang
Deng, Yiwei
A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title_full A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title_fullStr A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title_full_unstemmed A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title_short A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
title_sort rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605893/
https://www.ncbi.nlm.nih.gov/pubmed/28951669
http://dx.doi.org/10.1016/j.jsps.2017.01.002
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