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Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance
BACKGROUND: Microparticles (MPs) are vesicular structures shed from endothelial or circulating blood cells, after activation or apoptosis, and can be considered markers of vascular damage. We aimed to determine the levels of circulating MPs, their content of miRNA-126-3p and 5p, and their relationsh...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606070/ https://www.ncbi.nlm.nih.gov/pubmed/28927403 http://dx.doi.org/10.1186/s12933-017-0600-0 |
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author | Giannella, Alessandra Radu, Claudia Maria Franco, Lorenzo Campello, Elena Simioni, Paolo Avogaro, Angelo de Kreutzenberg, Saula Vigili Ceolotto, Giulio |
author_facet | Giannella, Alessandra Radu, Claudia Maria Franco, Lorenzo Campello, Elena Simioni, Paolo Avogaro, Angelo de Kreutzenberg, Saula Vigili Ceolotto, Giulio |
author_sort | Giannella, Alessandra |
collection | PubMed |
description | BACKGROUND: Microparticles (MPs) are vesicular structures shed from endothelial or circulating blood cells, after activation or apoptosis, and can be considered markers of vascular damage. We aimed to determine the levels of circulating MPs, their content of miRNA-126-3p and 5p, and their relationship with early endothelial activation/damage, in patients with different degree of glucose tolerance. METHODS: CD62E(+), CD62P(+), CD142(+), CD45(+) circulating MPs, their apoptotic (AnnexinV(+)) fractions, and miRNA-126 expression were determined in 39 prediabetic (PreDM), 68 type 2 diabetic (T2DM), and 53 control (NGT) subjects, along with main anthropometric and biochemical measurements. MPs were analysed by flow cytometry. miRNA-126 was measured by quantitative real-time PCR. Plasma antioxidant capacity was determined by electronic spin resonance; ICAM-1, and VCAM-1 by ELISA. RESULTS: Activated endothelial cell-derived MPs (CD62E(+)) were significantly increased in PreDM and T2DM in comparison to NGT (p < 0.0001). AnnexinV(+)/CD62E(+) MPs and Annexin V(+) MPs were significantly increased in T2DM compared to PreDM and NGT (p < 0.001); other MPs were not significantly different among groups. Plasma antioxidant capacity was significantly decreased in PreDM and T2DM compared to NGT (p = 0.001); VCAM-1 significantly increased in PreDM and T2DM in comparison to NGT (p = 0.001). miR-126-3p expression, but not miR-126-5p, in MPs, decreased significantly and progressively from NGT, to PreDM, and T2DM (p < 0.001). In PreDM and T2DM, CD62E(+) MPs level was significantly and negatively associated with plasma glucose (p = 0.004). CONCLUSION: We show for the first time that circulating CD62E(+) MPs level and miR-126-3p content in MPs are abnormal in subjects with pre-diabetes; the content of miR-126-3p correlates with markers of endothelial inflammation, such as VCAM-1, plasma antioxidant capacity, and microparticles, well-accepted markers of endothelial dysfunction. |
format | Online Article Text |
id | pubmed-5606070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56060702017-09-20 Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance Giannella, Alessandra Radu, Claudia Maria Franco, Lorenzo Campello, Elena Simioni, Paolo Avogaro, Angelo de Kreutzenberg, Saula Vigili Ceolotto, Giulio Cardiovasc Diabetol Original Investigation BACKGROUND: Microparticles (MPs) are vesicular structures shed from endothelial or circulating blood cells, after activation or apoptosis, and can be considered markers of vascular damage. We aimed to determine the levels of circulating MPs, their content of miRNA-126-3p and 5p, and their relationship with early endothelial activation/damage, in patients with different degree of glucose tolerance. METHODS: CD62E(+), CD62P(+), CD142(+), CD45(+) circulating MPs, their apoptotic (AnnexinV(+)) fractions, and miRNA-126 expression were determined in 39 prediabetic (PreDM), 68 type 2 diabetic (T2DM), and 53 control (NGT) subjects, along with main anthropometric and biochemical measurements. MPs were analysed by flow cytometry. miRNA-126 was measured by quantitative real-time PCR. Plasma antioxidant capacity was determined by electronic spin resonance; ICAM-1, and VCAM-1 by ELISA. RESULTS: Activated endothelial cell-derived MPs (CD62E(+)) were significantly increased in PreDM and T2DM in comparison to NGT (p < 0.0001). AnnexinV(+)/CD62E(+) MPs and Annexin V(+) MPs were significantly increased in T2DM compared to PreDM and NGT (p < 0.001); other MPs were not significantly different among groups. Plasma antioxidant capacity was significantly decreased in PreDM and T2DM compared to NGT (p = 0.001); VCAM-1 significantly increased in PreDM and T2DM in comparison to NGT (p = 0.001). miR-126-3p expression, but not miR-126-5p, in MPs, decreased significantly and progressively from NGT, to PreDM, and T2DM (p < 0.001). In PreDM and T2DM, CD62E(+) MPs level was significantly and negatively associated with plasma glucose (p = 0.004). CONCLUSION: We show for the first time that circulating CD62E(+) MPs level and miR-126-3p content in MPs are abnormal in subjects with pre-diabetes; the content of miR-126-3p correlates with markers of endothelial inflammation, such as VCAM-1, plasma antioxidant capacity, and microparticles, well-accepted markers of endothelial dysfunction. BioMed Central 2017-09-19 /pmc/articles/PMC5606070/ /pubmed/28927403 http://dx.doi.org/10.1186/s12933-017-0600-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Original Investigation Giannella, Alessandra Radu, Claudia Maria Franco, Lorenzo Campello, Elena Simioni, Paolo Avogaro, Angelo de Kreutzenberg, Saula Vigili Ceolotto, Giulio Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title | Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title_full | Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title_fullStr | Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title_full_unstemmed | Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title_short | Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
title_sort | circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606070/ https://www.ncbi.nlm.nih.gov/pubmed/28927403 http://dx.doi.org/10.1186/s12933-017-0600-0 |
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