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Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays
The biological effects of low-dose or low-dose-rate ionizing radiation on normal tissues has attracted attention. Based on previous research, we observed the morphology of liver tissues of C57BL/6J mice that received <50, 50–500, and 500–1000 μGy/h of (137)Cs radiation for 180 d. We found that th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607120/ https://www.ncbi.nlm.nih.gov/pubmed/28931006 http://dx.doi.org/10.1371/journal.pone.0182671 |
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author | Yi, Lan Hu, Nan Yin, Jie Sun, Jing Mu, Hongxiang Dai, Keren Ding, Dexin |
author_facet | Yi, Lan Hu, Nan Yin, Jie Sun, Jing Mu, Hongxiang Dai, Keren Ding, Dexin |
author_sort | Yi, Lan |
collection | PubMed |
description | The biological effects of low-dose or low-dose-rate ionizing radiation on normal tissues has attracted attention. Based on previous research, we observed the morphology of liver tissues of C57BL/6J mice that received <50, 50–500, and 500–1000 μGy/h of (137)Cs radiation for 180 d. We found that the pathological changes in liver tissues were more obvious as the irradiation dose rates increased. Additionally, differential protein expression in liver tissues was analyzed using a proteomics approach. Compared with the matched group in the 2D gel analysis of the irradiated groups, 69 proteins had ≥ 1.5-fold changes in expression. Twenty-three proteins were selected based on ≥2.5-fold change in expression, and 22 of them were meaningful for bioinformatics and protein fingerprinting analysis. These molecules were relevant to cytoskeleton processes, cell metabolism, biological defense, mitochondrial damage, detoxification and tumorigenesis. The results from real-time PCR and western blot (WB) analyses showed that calreticulin (CRT) was up-regulated in the irradiated groups, which indicates that CRT may be relevant to stress reactions when mouse livers are exposed to low-dose irradiation and that low-dose-rate ionizing radiation may pose a cancer risk. The CRT protein can be a potential candidate for low-dose or low-dose-rate ionizing radiation early-warning biomarkers. However, the underlying mechanism requires further investigation. |
format | Online Article Text |
id | pubmed-5607120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56071202017-10-09 Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays Yi, Lan Hu, Nan Yin, Jie Sun, Jing Mu, Hongxiang Dai, Keren Ding, Dexin PLoS One Research Article The biological effects of low-dose or low-dose-rate ionizing radiation on normal tissues has attracted attention. Based on previous research, we observed the morphology of liver tissues of C57BL/6J mice that received <50, 50–500, and 500–1000 μGy/h of (137)Cs radiation for 180 d. We found that the pathological changes in liver tissues were more obvious as the irradiation dose rates increased. Additionally, differential protein expression in liver tissues was analyzed using a proteomics approach. Compared with the matched group in the 2D gel analysis of the irradiated groups, 69 proteins had ≥ 1.5-fold changes in expression. Twenty-three proteins were selected based on ≥2.5-fold change in expression, and 22 of them were meaningful for bioinformatics and protein fingerprinting analysis. These molecules were relevant to cytoskeleton processes, cell metabolism, biological defense, mitochondrial damage, detoxification and tumorigenesis. The results from real-time PCR and western blot (WB) analyses showed that calreticulin (CRT) was up-regulated in the irradiated groups, which indicates that CRT may be relevant to stress reactions when mouse livers are exposed to low-dose irradiation and that low-dose-rate ionizing radiation may pose a cancer risk. The CRT protein can be a potential candidate for low-dose or low-dose-rate ionizing radiation early-warning biomarkers. However, the underlying mechanism requires further investigation. Public Library of Science 2017-09-20 /pmc/articles/PMC5607120/ /pubmed/28931006 http://dx.doi.org/10.1371/journal.pone.0182671 Text en © 2017 Yi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yi, Lan Hu, Nan Yin, Jie Sun, Jing Mu, Hongxiang Dai, Keren Ding, Dexin Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title | Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title_full | Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title_fullStr | Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title_full_unstemmed | Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title_short | Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
title_sort | up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607120/ https://www.ncbi.nlm.nih.gov/pubmed/28931006 http://dx.doi.org/10.1371/journal.pone.0182671 |
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