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Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands

In recent years, studies related to the expression profiles of miRNAs in the dairy goat mammary gland were performed, but regulatory mechanisms in the physiological environment and the dynamic homeostasis of mammary gland development and lactation are not clear. In the present study, sequencing data...

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Autores principales: Ji, Zhibin, Liu, Zhaohua, Chao, Tianle, Hou, Lei, Fan, Rui, He, Rongyan, Wang, Guizhi, Wang, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607250/
https://www.ncbi.nlm.nih.gov/pubmed/28931951
http://dx.doi.org/10.1038/s41598-017-12297-4
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author Ji, Zhibin
Liu, Zhaohua
Chao, Tianle
Hou, Lei
Fan, Rui
He, Rongyan
Wang, Guizhi
Wang, Jianmin
author_facet Ji, Zhibin
Liu, Zhaohua
Chao, Tianle
Hou, Lei
Fan, Rui
He, Rongyan
Wang, Guizhi
Wang, Jianmin
author_sort Ji, Zhibin
collection PubMed
description In recent years, studies related to the expression profiles of miRNAs in the dairy goat mammary gland were performed, but regulatory mechanisms in the physiological environment and the dynamic homeostasis of mammary gland development and lactation are not clear. In the present study, sequencing data analysis of early and late lactation uncovered a total of 1,487 unique miRNAs, including 45 novel miRNA candidates and 1,442 known and conserved miRNAs, of which 758 miRNAs were co-expressed and 378 differentially expressed with P < 0.05. Moreover, 76 non-redundant target genes were annotated in 347 GO consortiums, with 3,143 candidate target genes grouped into 33 pathways. Additionally, 18 predicted target genes of 214 miRNAs were directly annotated in mammary gland development and used to construct regulatory networks based on GO annotation and the KEGG pathway. The expression levels of seven known miRNAs and three novel miRNAs were examined using quantitative real-time PCR. The results showed that miRNAs might play important roles in early and late lactation during dairy goat mammary gland development, which will be helpful to obtain a better understanding of the genetic control of mammary gland lactation and development.
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spelling pubmed-56072502017-09-24 Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands Ji, Zhibin Liu, Zhaohua Chao, Tianle Hou, Lei Fan, Rui He, Rongyan Wang, Guizhi Wang, Jianmin Sci Rep Article In recent years, studies related to the expression profiles of miRNAs in the dairy goat mammary gland were performed, but regulatory mechanisms in the physiological environment and the dynamic homeostasis of mammary gland development and lactation are not clear. In the present study, sequencing data analysis of early and late lactation uncovered a total of 1,487 unique miRNAs, including 45 novel miRNA candidates and 1,442 known and conserved miRNAs, of which 758 miRNAs were co-expressed and 378 differentially expressed with P < 0.05. Moreover, 76 non-redundant target genes were annotated in 347 GO consortiums, with 3,143 candidate target genes grouped into 33 pathways. Additionally, 18 predicted target genes of 214 miRNAs were directly annotated in mammary gland development and used to construct regulatory networks based on GO annotation and the KEGG pathway. The expression levels of seven known miRNAs and three novel miRNAs were examined using quantitative real-time PCR. The results showed that miRNAs might play important roles in early and late lactation during dairy goat mammary gland development, which will be helpful to obtain a better understanding of the genetic control of mammary gland lactation and development. Nature Publishing Group UK 2017-09-20 /pmc/articles/PMC5607250/ /pubmed/28931951 http://dx.doi.org/10.1038/s41598-017-12297-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ji, Zhibin
Liu, Zhaohua
Chao, Tianle
Hou, Lei
Fan, Rui
He, Rongyan
Wang, Guizhi
Wang, Jianmin
Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title_full Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title_fullStr Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title_full_unstemmed Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title_short Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
title_sort screening of mirna profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607250/
https://www.ncbi.nlm.nih.gov/pubmed/28931951
http://dx.doi.org/10.1038/s41598-017-12297-4
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