Cargando…

Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria

PURPOSE: Detection of carbapenemases among Gram-negative bacteria (GNB) is important for both clinicians and infection control practitioners. The Clinical and Laboratory Standards Institute recommends Carba NP (CNP) as confirmatory test for carbapenemase production. The reagents required for CNP tes...

Descripción completa

Detalles Bibliográficos
Autores principales: Rudresh, Shoorashetty Manohara, Ravi, Giriyapur Siddappa, Sunitha, Lakshminarayanappa, Hajira, Sadiya Noor, Kalaiarasan, Ellappan, Harish, Belgode Narasimha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607762/
https://www.ncbi.nlm.nih.gov/pubmed/28966495
http://dx.doi.org/10.4103/JLP.JLP_138_16
_version_ 1783265333617885184
author Rudresh, Shoorashetty Manohara
Ravi, Giriyapur Siddappa
Sunitha, Lakshminarayanappa
Hajira, Sadiya Noor
Kalaiarasan, Ellappan
Harish, Belgode Narasimha
author_facet Rudresh, Shoorashetty Manohara
Ravi, Giriyapur Siddappa
Sunitha, Lakshminarayanappa
Hajira, Sadiya Noor
Kalaiarasan, Ellappan
Harish, Belgode Narasimha
author_sort Rudresh, Shoorashetty Manohara
collection PubMed
description PURPOSE: Detection of carbapenemases among Gram-negative bacteria (GNB) is important for both clinicians and infection control practitioners. The Clinical and Laboratory Standards Institute recommends Carba NP (CNP) as confirmatory test for carbapenemase production. The reagents required for CNP test are costly and hence the test cannot be performed on a routine basis. The present study evaluates modifications of CNP test for rapid detection of carbapenemases among GNB. MATERIALS AND METHODS: The GNB were screened for carbapenemase production using CNP, CarbAcineto NP (CANP), and modified CNP (mCNP) test. A multiplex polymerase chain reaction (PCR) was performed on all the carbapenem-resistant bacteria for carbapenemase genes. The results of three phenotypic tests were compared with PCR. RESULTS: A total of 765 gram negative bacteria were screened for carbapenem resistance. Carbapenem resistance was found in 144 GNB. The metallo-β-lactamases were most common carbapenemases followed by OXA-48-like enzymes. The CANP test was most sensitive (80.6%) for carbapenemases detection. The mCNP test was 62.1% sensitive for detection of carbapenemases. The mCNP, CNP, and CANP tests were equally sensitive (95%) for detection of NDM enzymes among Enterobacteriaceae. The mCNP test had poor sensitivity for detection of OXA-48-like enzymes. CONCLUSION: The mCNP test was rapid, cost-effective, and easily adoptable on routine basis. The early detection of carbapenemases using mCNP test will help in preventing the spread of multidrug-resistant organisms in the hospital settings.
format Online
Article
Text
id pubmed-5607762
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-56077622017-10-01 Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria Rudresh, Shoorashetty Manohara Ravi, Giriyapur Siddappa Sunitha, Lakshminarayanappa Hajira, Sadiya Noor Kalaiarasan, Ellappan Harish, Belgode Narasimha J Lab Physicians Original Article PURPOSE: Detection of carbapenemases among Gram-negative bacteria (GNB) is important for both clinicians and infection control practitioners. The Clinical and Laboratory Standards Institute recommends Carba NP (CNP) as confirmatory test for carbapenemase production. The reagents required for CNP test are costly and hence the test cannot be performed on a routine basis. The present study evaluates modifications of CNP test for rapid detection of carbapenemases among GNB. MATERIALS AND METHODS: The GNB were screened for carbapenemase production using CNP, CarbAcineto NP (CANP), and modified CNP (mCNP) test. A multiplex polymerase chain reaction (PCR) was performed on all the carbapenem-resistant bacteria for carbapenemase genes. The results of three phenotypic tests were compared with PCR. RESULTS: A total of 765 gram negative bacteria were screened for carbapenem resistance. Carbapenem resistance was found in 144 GNB. The metallo-β-lactamases were most common carbapenemases followed by OXA-48-like enzymes. The CANP test was most sensitive (80.6%) for carbapenemases detection. The mCNP test was 62.1% sensitive for detection of carbapenemases. The mCNP, CNP, and CANP tests were equally sensitive (95%) for detection of NDM enzymes among Enterobacteriaceae. The mCNP test had poor sensitivity for detection of OXA-48-like enzymes. CONCLUSION: The mCNP test was rapid, cost-effective, and easily adoptable on routine basis. The early detection of carbapenemases using mCNP test will help in preventing the spread of multidrug-resistant organisms in the hospital settings. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5607762/ /pubmed/28966495 http://dx.doi.org/10.4103/JLP.JLP_138_16 Text en Copyright: © 2017 Journal of Laboratory Physicians http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rudresh, Shoorashetty Manohara
Ravi, Giriyapur Siddappa
Sunitha, Lakshminarayanappa
Hajira, Sadiya Noor
Kalaiarasan, Ellappan
Harish, Belgode Narasimha
Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title_full Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title_fullStr Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title_full_unstemmed Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title_short Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria
title_sort simple, rapid, and cost-effective modified carba np test for carbapenemase detection among gram-negative bacteria
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607762/
https://www.ncbi.nlm.nih.gov/pubmed/28966495
http://dx.doi.org/10.4103/JLP.JLP_138_16
work_keys_str_mv AT rudreshshoorashettymanohara simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria
AT ravigiriyapursiddappa simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria
AT sunithalakshminarayanappa simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria
AT hajirasadiyanoor simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria
AT kalaiarasanellappan simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria
AT harishbelgodenarasimha simplerapidandcosteffectivemodifiedcarbanptestforcarbapenemasedetectionamonggramnegativebacteria