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A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord

Non-traumatic injury accounts for approximately half of clinical spinal cord injury, including chronic spinal cord compression. However, previous rodent spinal cord compression models are mainly designed for rats, few are available for mice. Our aim is to develop a thoracic progressive compression m...

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Autores principales: Sun, Guo-dong, Chen, Yan, Zhou, Zhi-gang, Yang, Shu-xian, Zhong, Cheng, Li, Zhi-zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607834/
https://www.ncbi.nlm.nih.gov/pubmed/28966654
http://dx.doi.org/10.4103/1673-5374.213693
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author Sun, Guo-dong
Chen, Yan
Zhou, Zhi-gang
Yang, Shu-xian
Zhong, Cheng
Li, Zhi-zhong
author_facet Sun, Guo-dong
Chen, Yan
Zhou, Zhi-gang
Yang, Shu-xian
Zhong, Cheng
Li, Zhi-zhong
author_sort Sun, Guo-dong
collection PubMed
description Non-traumatic injury accounts for approximately half of clinical spinal cord injury, including chronic spinal cord compression. However, previous rodent spinal cord compression models are mainly designed for rats, few are available for mice. Our aim is to develop a thoracic progressive compression mice model of spinal cord injury. In this study, adult wild-type C57BL/6 mice were divided into two groups: in the surgery group, a screw was inserted at T(9) lamina to compress the spinal cord, and the compression was increased by turning it further into the canal (0.2 mm) post-surgery every 2 weeks up to 8 weeks. In the control group, a hole was drilled into the lamina without inserting a screw. The results showed that Basso Mouse Scale scores were lower and gait worsened. In addition, the degree of hindlimb dysfunction in mice was consistent with the degree of spinal cord compression. The number of motor neurons in the anterior horn of the spinal cord was reduced in all groups of mice, whereas astrocytes and microglia were gradually activated and proliferated. In conclusion, this progressive compression of thoracic spinal cord injury in mice is a preferable model for chronic progressive spinal cord compression injury.
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spelling pubmed-56078342017-09-29 A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord Sun, Guo-dong Chen, Yan Zhou, Zhi-gang Yang, Shu-xian Zhong, Cheng Li, Zhi-zhong Neural Regen Res Research Article Non-traumatic injury accounts for approximately half of clinical spinal cord injury, including chronic spinal cord compression. However, previous rodent spinal cord compression models are mainly designed for rats, few are available for mice. Our aim is to develop a thoracic progressive compression mice model of spinal cord injury. In this study, adult wild-type C57BL/6 mice were divided into two groups: in the surgery group, a screw was inserted at T(9) lamina to compress the spinal cord, and the compression was increased by turning it further into the canal (0.2 mm) post-surgery every 2 weeks up to 8 weeks. In the control group, a hole was drilled into the lamina without inserting a screw. The results showed that Basso Mouse Scale scores were lower and gait worsened. In addition, the degree of hindlimb dysfunction in mice was consistent with the degree of spinal cord compression. The number of motor neurons in the anterior horn of the spinal cord was reduced in all groups of mice, whereas astrocytes and microglia were gradually activated and proliferated. In conclusion, this progressive compression of thoracic spinal cord injury in mice is a preferable model for chronic progressive spinal cord compression injury. Medknow Publications & Media Pvt Ltd 2017-08 /pmc/articles/PMC5607834/ /pubmed/28966654 http://dx.doi.org/10.4103/1673-5374.213693 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Sun, Guo-dong
Chen, Yan
Zhou, Zhi-gang
Yang, Shu-xian
Zhong, Cheng
Li, Zhi-zhong
A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title_full A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title_fullStr A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title_full_unstemmed A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title_short A progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
title_sort progressive compression model of thoracic spinal cord injury in mice: function assessment and pathological changes in spinal cord
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607834/
https://www.ncbi.nlm.nih.gov/pubmed/28966654
http://dx.doi.org/10.4103/1673-5374.213693
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