GuideScan software for improved single and paired CRISPR guide RNA design

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of gRNAs for single- and paired-gRNA genome-wide screens. We also show that by using a trie data structure GuideScan designs...

Descripción completa

Detalles Bibliográficos
Autores principales: Perez, Alexendar R., Pritykin, Yuri, Vidigal, Joana A., Chhangawala, Sagar, Zamparo, Lee, Leslie, Christina S., Ventura, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607865/
https://www.ncbi.nlm.nih.gov/pubmed/28263296
http://dx.doi.org/10.1038/nbt.3804
_version_ 1783265355067555840
author Perez, Alexendar R.
Pritykin, Yuri
Vidigal, Joana A.
Chhangawala, Sagar
Zamparo, Lee
Leslie, Christina S.
Ventura, Andrea
author_facet Perez, Alexendar R.
Pritykin, Yuri
Vidigal, Joana A.
Chhangawala, Sagar
Zamparo, Lee
Leslie, Christina S.
Ventura, Andrea
author_sort Perez, Alexendar R.
collection PubMed
description We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of gRNAs for single- and paired-gRNA genome-wide screens. We also show that by using a trie data structure GuideScan designs gRNAs that are more specific than those designed by existing tools.
format Online
Article
Text
id pubmed-5607865
institution National Center for Biotechnology Information
language English
publishDate 2017
record_format MEDLINE/PubMed
spelling pubmed-56078652017-09-22 GuideScan software for improved single and paired CRISPR guide RNA design Perez, Alexendar R. Pritykin, Yuri Vidigal, Joana A. Chhangawala, Sagar Zamparo, Lee Leslie, Christina S. Ventura, Andrea Nat Biotechnol Article We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of gRNAs for single- and paired-gRNA genome-wide screens. We also show that by using a trie data structure GuideScan designs gRNAs that are more specific than those designed by existing tools. 2017-03-06 2017-04 /pmc/articles/PMC5607865/ /pubmed/28263296 http://dx.doi.org/10.1038/nbt.3804 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Perez, Alexendar R.
Pritykin, Yuri
Vidigal, Joana A.
Chhangawala, Sagar
Zamparo, Lee
Leslie, Christina S.
Ventura, Andrea
GuideScan software for improved single and paired CRISPR guide RNA design
title GuideScan software for improved single and paired CRISPR guide RNA design
title_full GuideScan software for improved single and paired CRISPR guide RNA design
title_fullStr GuideScan software for improved single and paired CRISPR guide RNA design
title_full_unstemmed GuideScan software for improved single and paired CRISPR guide RNA design
title_short GuideScan software for improved single and paired CRISPR guide RNA design
title_sort guidescan software for improved single and paired crispr guide rna design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607865/
https://www.ncbi.nlm.nih.gov/pubmed/28263296
http://dx.doi.org/10.1038/nbt.3804
work_keys_str_mv AT perezalexendarr guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT pritykinyuri guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT vidigaljoanaa guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT chhangawalasagar guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT zamparolee guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT lesliechristinas guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign
AT venturaandrea guidescansoftwareforimprovedsingleandpairedcrisprguidernadesign

Ejemplares similares