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Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan

OBJECTIVES: To address the lack of up-to-date published data, the present study evaluates the PCR-based prevalence of Cryptosporidium species infection and molecular characteristics of isolates among household cats and pet shop kittens in Japan. METHODS: A total of 357 and 329 fresh faecal samples w...

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Autores principales: Ito, Yoichi, Itoh, Naoyuki, Iijima, Yuko, Kimura, Yuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607915/
https://www.ncbi.nlm.nih.gov/pubmed/28955478
http://dx.doi.org/10.1177/2055116917730719
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author Ito, Yoichi
Itoh, Naoyuki
Iijima, Yuko
Kimura, Yuya
author_facet Ito, Yoichi
Itoh, Naoyuki
Iijima, Yuko
Kimura, Yuya
author_sort Ito, Yoichi
collection PubMed
description OBJECTIVES: To address the lack of up-to-date published data, the present study evaluates the PCR-based prevalence of Cryptosporidium species infection and molecular characteristics of isolates among household cats and pet shop kittens in Japan. METHODS: A total of 357 and 329 fresh faecal samples were collected from household cats and pet shop kittens, respectively, with or without clinical signs of infection. A nested PCR assay targeting the 18S rRNA gene was employed for the detection of Cryptosporidium species. After specific DNA fragments (approximately 826 base pairs) were confirmed, the amplicons were sequenced to determine species. RESULTS: Seven (2.0%) household cats and one (0.3%) pet shop kitten tested positive for the presence of Cryptosporidium species. In household cats, there was a significant difference in prevalence between cats aged <1 year (4.6%) and those aged ⩾1 year (0.4%). No significantly different prevalence was observed with regard to faecal condition in either household cats or pet shop kittens. A total of eight Cryptosporidium species isolates, seven from household cats and one from a pet shop kitten, were identified as Cryptosporidium felis. CONCLUSIONS AND RELEVANCE: The present study demonstrates the risk of zoonotic transmission of Cryptosporidium species from household cats and pet shop kittens to humans is low in Japan.
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spelling pubmed-56079152017-09-27 Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan Ito, Yoichi Itoh, Naoyuki Iijima, Yuko Kimura, Yuya JFMS Open Rep Short Communication OBJECTIVES: To address the lack of up-to-date published data, the present study evaluates the PCR-based prevalence of Cryptosporidium species infection and molecular characteristics of isolates among household cats and pet shop kittens in Japan. METHODS: A total of 357 and 329 fresh faecal samples were collected from household cats and pet shop kittens, respectively, with or without clinical signs of infection. A nested PCR assay targeting the 18S rRNA gene was employed for the detection of Cryptosporidium species. After specific DNA fragments (approximately 826 base pairs) were confirmed, the amplicons were sequenced to determine species. RESULTS: Seven (2.0%) household cats and one (0.3%) pet shop kitten tested positive for the presence of Cryptosporidium species. In household cats, there was a significant difference in prevalence between cats aged <1 year (4.6%) and those aged ⩾1 year (0.4%). No significantly different prevalence was observed with regard to faecal condition in either household cats or pet shop kittens. A total of eight Cryptosporidium species isolates, seven from household cats and one from a pet shop kitten, were identified as Cryptosporidium felis. CONCLUSIONS AND RELEVANCE: The present study demonstrates the risk of zoonotic transmission of Cryptosporidium species from household cats and pet shop kittens to humans is low in Japan. SAGE Publications 2017-09-19 /pmc/articles/PMC5607915/ /pubmed/28955478 http://dx.doi.org/10.1177/2055116917730719 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Short Communication
Ito, Yoichi
Itoh, Naoyuki
Iijima, Yuko
Kimura, Yuya
Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title_full Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title_fullStr Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title_full_unstemmed Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title_short Molecular prevalence of Cryptosporidium species among household cats and pet shop kittens in Japan
title_sort molecular prevalence of cryptosporidium species among household cats and pet shop kittens in japan
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607915/
https://www.ncbi.nlm.nih.gov/pubmed/28955478
http://dx.doi.org/10.1177/2055116917730719
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