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Measuring Protein Binding to F-actin by Co-sedimentation

Filamentous actin (F-actin) organization within cells is regulated by a large number of actin-binding proteins that control actin nucleation, growth, cross-linking and/or disassembly. This protocol describes a technique – the actin co-sedimentation, or pelleting, assay – to determine whether a prote...

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Detalles Bibliográficos
Autores principales:	Heier, Jonathon A., Dickinson, Daniel J., Kwiatkowski, Adam V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2017
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607963/ https://www.ncbi.nlm.nih.gov/pubmed/28570520 http://dx.doi.org/10.3791/55613

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