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Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Diabetic Nephropathy Prevention
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607974/ https://www.ncbi.nlm.nih.gov/pubmed/28975093 http://dx.doi.org/10.15171/jnp.2017.24 |
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author | Hashemi, Mohammad Sadeghi-Bojd, Simin Aryanezhad, Soheib Rezaei, Maryam |
author_facet | Hashemi, Mohammad Sadeghi-Bojd, Simin Aryanezhad, Soheib Rezaei, Maryam |
author_sort | Hashemi, Mohammad |
collection | PubMed |
description | BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. RESULTS: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. CONCLUSIONS: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings. |
format | Online Article Text |
id | pubmed-5607974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Society of Diabetic Nephropathy Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-56079742017-10-03 Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children Hashemi, Mohammad Sadeghi-Bojd, Simin Aryanezhad, Soheib Rezaei, Maryam J Nephropathol Original Article BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. RESULTS: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. CONCLUSIONS: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings. Society of Diabetic Nephropathy Prevention 2017-07 2016-12-17 /pmc/articles/PMC5607974/ /pubmed/28975093 http://dx.doi.org/10.15171/jnp.2017.24 Text en © 2017 The Author(s) Published by Society of Diabetic Nephropathy Prevention. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Hashemi, Mohammad Sadeghi-Bojd, Simin Aryanezhad, Soheib Rezaei, Maryam Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title | Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title_full | Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title_fullStr | Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title_full_unstemmed | Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title_short | Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children |
title_sort | association of endothelin-1 rs5370 g>t gene polymorphism with the risk of nephrotic syndrome in children |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607974/ https://www.ncbi.nlm.nih.gov/pubmed/28975093 http://dx.doi.org/10.15171/jnp.2017.24 |
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