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Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children

BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study w...

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Autores principales: Hashemi, Mohammad, Sadeghi-Bojd, Simin, Aryanezhad, Soheib, Rezaei, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Diabetic Nephropathy Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607974/
https://www.ncbi.nlm.nih.gov/pubmed/28975093
http://dx.doi.org/10.15171/jnp.2017.24
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author Hashemi, Mohammad
Sadeghi-Bojd, Simin
Aryanezhad, Soheib
Rezaei, Maryam
author_facet Hashemi, Mohammad
Sadeghi-Bojd, Simin
Aryanezhad, Soheib
Rezaei, Maryam
author_sort Hashemi, Mohammad
collection PubMed
description BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. RESULTS: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. CONCLUSIONS: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings.
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spelling pubmed-56079742017-10-03 Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children Hashemi, Mohammad Sadeghi-Bojd, Simin Aryanezhad, Soheib Rezaei, Maryam J Nephropathol Original Article BACKGROUND: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. PATIENTS AND METHODS: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. RESULTS: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. CONCLUSIONS: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings. Society of Diabetic Nephropathy Prevention 2017-07 2016-12-17 /pmc/articles/PMC5607974/ /pubmed/28975093 http://dx.doi.org/10.15171/jnp.2017.24 Text en © 2017 The Author(s) Published by Society of Diabetic Nephropathy Prevention. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hashemi, Mohammad
Sadeghi-Bojd, Simin
Aryanezhad, Soheib
Rezaei, Maryam
Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title_full Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title_fullStr Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title_full_unstemmed Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title_short Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
title_sort association of endothelin-1 rs5370 g>t gene polymorphism with the risk of nephrotic syndrome in children
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607974/
https://www.ncbi.nlm.nih.gov/pubmed/28975093
http://dx.doi.org/10.15171/jnp.2017.24
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