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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

SPHIRE (SPARX for High-Resolution Electron Microscopy) is a novel open-source, user-friendly software suite for the semi-automated processing of single particle electron cryo-microscopy (cryo-EM) data. The protocol presented here describes in detail how to obtain a near-atomic resolution structure s...

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Detalles Bibliográficos
Autores principales:	Moriya, Toshio, Saur, Michael, Stabrin, Markus, Merino, Felipe, Voicu, Horatiu, Huang, Zhong, Penczek, Pawel A., Raunser, Stefan, Gatsogiannis, Christos
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2017
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607996/ https://www.ncbi.nlm.nih.gov/pubmed/28570515 http://dx.doi.org/10.3791/55448

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