Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS

We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obt...

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Detalles Bibliográficos
Autores principales: Arca-Suárez, Jorge, Galán-Sánchez, Fátima, Marin-Casanova, Pilar, Rodríguez-Iglesias, Manuel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608331/
https://www.ncbi.nlm.nih.gov/pubmed/28934331
http://dx.doi.org/10.1371/journal.pone.0185229
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author Arca-Suárez, Jorge
Galán-Sánchez, Fátima
Marin-Casanova, Pilar
Rodríguez-Iglesias, Manuel A.
author_facet Arca-Suárez, Jorge
Galán-Sánchez, Fátima
Marin-Casanova, Pilar
Rodríguez-Iglesias, Manuel A.
author_sort Arca-Suárez, Jorge
collection PubMed
description We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
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spelling pubmed-56083312017-10-09 Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS Arca-Suárez, Jorge Galán-Sánchez, Fátima Marin-Casanova, Pilar Rodríguez-Iglesias, Manuel A. PLoS One Research Article We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories. Public Library of Science 2017-09-21 /pmc/articles/PMC5608331/ /pubmed/28934331 http://dx.doi.org/10.1371/journal.pone.0185229 Text en © 2017 Arca-Suárez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Arca-Suárez, Jorge
Galán-Sánchez, Fátima
Marin-Casanova, Pilar
Rodríguez-Iglesias, Manuel A.
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title_full Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title_fullStr Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title_full_unstemmed Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title_short Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
title_sort direct identification of microorganisms from thioglycolate broth by maldi-tof ms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608331/
https://www.ncbi.nlm.nih.gov/pubmed/28934331
http://dx.doi.org/10.1371/journal.pone.0185229
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