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Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples

The study was conducted to select the best promising keratinolytic bacterial strain. A good keratinase positive bacterium isolated from the soil samples of Hazaribagh tannery industrial zone, Dhaka was identified as Arthrobacter genus depending on the conventional techniques and confirmed as Arthrob...

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Autores principales: Barman, Nirmal Chandra, Zohora, Fatema Tuj, Das, Keshob Chandra, Mowla, Md. Golam, Banu, Nilufa Akhter, Salimullah, Md., Hashem, Abu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608654/
https://www.ncbi.nlm.nih.gov/pubmed/28936604
http://dx.doi.org/10.1186/s13568-017-0462-6
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author Barman, Nirmal Chandra
Zohora, Fatema Tuj
Das, Keshob Chandra
Mowla, Md. Golam
Banu, Nilufa Akhter
Salimullah, Md.
Hashem, Abu
author_facet Barman, Nirmal Chandra
Zohora, Fatema Tuj
Das, Keshob Chandra
Mowla, Md. Golam
Banu, Nilufa Akhter
Salimullah, Md.
Hashem, Abu
author_sort Barman, Nirmal Chandra
collection PubMed
description The study was conducted to select the best promising keratinolytic bacterial strain. A good keratinase positive bacterium isolated from the soil samples of Hazaribagh tannery industrial zone, Dhaka was identified as Arthrobacter genus depending on the conventional techniques and confirmed as Arthrobacter sp. by sequencing 16S rRNA gene. The medium components and culture conditions were optimized to enhance keratinase production through shake flask culture. Keratin and feather powder (10 g/l or 1%) were good substrates for the highest keratinase production along with yeast extract (0.2 g/l or 0.02%) as an organic nitrogen source and potassium nitrate (1 g or 0.1%) as an inorganic nitrogen source. Maximum yield of keratinase was found after 24 h of incubation at 37 °C with an initial pH of 7.0 and inoculums volume 5% under 150 rpm when keratin, yeast extract and potassium nitrate were used as nutrient sources. Keratinase production was more than 5.0-fold increased when all optimized parameters were applied simultaneously. The optimum reaction temperature and pH were determined to be 40 °C and 8.0 respectively for crude keratinase activity. Therefore, Arthrobacter sp. NFH5 might be used for large scale production of keratinase for industrial purposes in less time. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-017-0462-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-56086542017-10-10 Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples Barman, Nirmal Chandra Zohora, Fatema Tuj Das, Keshob Chandra Mowla, Md. Golam Banu, Nilufa Akhter Salimullah, Md. Hashem, Abu AMB Express Original Article The study was conducted to select the best promising keratinolytic bacterial strain. A good keratinase positive bacterium isolated from the soil samples of Hazaribagh tannery industrial zone, Dhaka was identified as Arthrobacter genus depending on the conventional techniques and confirmed as Arthrobacter sp. by sequencing 16S rRNA gene. The medium components and culture conditions were optimized to enhance keratinase production through shake flask culture. Keratin and feather powder (10 g/l or 1%) were good substrates for the highest keratinase production along with yeast extract (0.2 g/l or 0.02%) as an organic nitrogen source and potassium nitrate (1 g or 0.1%) as an inorganic nitrogen source. Maximum yield of keratinase was found after 24 h of incubation at 37 °C with an initial pH of 7.0 and inoculums volume 5% under 150 rpm when keratin, yeast extract and potassium nitrate were used as nutrient sources. Keratinase production was more than 5.0-fold increased when all optimized parameters were applied simultaneously. The optimum reaction temperature and pH were determined to be 40 °C and 8.0 respectively for crude keratinase activity. Therefore, Arthrobacter sp. NFH5 might be used for large scale production of keratinase for industrial purposes in less time. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-017-0462-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-09-21 /pmc/articles/PMC5608654/ /pubmed/28936604 http://dx.doi.org/10.1186/s13568-017-0462-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Barman, Nirmal Chandra
Zohora, Fatema Tuj
Das, Keshob Chandra
Mowla, Md. Golam
Banu, Nilufa Akhter
Salimullah, Md.
Hashem, Abu
Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title_full Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title_fullStr Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title_full_unstemmed Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title_short Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples
title_sort production, partial optimization and characterization of keratinase enzyme by arthrobacter sp. nfh5 isolated from soil samples
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608654/
https://www.ncbi.nlm.nih.gov/pubmed/28936604
http://dx.doi.org/10.1186/s13568-017-0462-6
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