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PD-L1 is an activation-independent marker of brown adipocytes

Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-...

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Detalles Bibliográficos
Autores principales: Ingram, Jessica R., Dougan, Michael, Rashidian, Mohammad, Knoll, Marko, Keliher, Edmund J., Garrett, Sarah, Garforth, Scott, Blomberg, Olga S., Espinosa, Camilo, Bhan, Atul, Almo, Steven C., Weissleder, Ralph, Lodish, Harvey, Dougan, Stephanie K., Ploegh, Hidde L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608754/
https://www.ncbi.nlm.nih.gov/pubmed/28935898
http://dx.doi.org/10.1038/s41467-017-00799-8
Descripción
Sumario:Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.