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PD-L1 is an activation-independent marker of brown adipocytes
Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608754/ https://www.ncbi.nlm.nih.gov/pubmed/28935898 http://dx.doi.org/10.1038/s41467-017-00799-8 |
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author | Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Lodish, Harvey Dougan, Stephanie K. Ploegh, Hidde L. |
author_facet | Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Lodish, Harvey Dougan, Stephanie K. Ploegh, Hidde L. |
author_sort | Ingram, Jessica R. |
collection | PubMed |
description | Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. |
format | Online Article Text |
id | pubmed-5608754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56087542017-09-25 PD-L1 is an activation-independent marker of brown adipocytes Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Lodish, Harvey Dougan, Stephanie K. Ploegh, Hidde L. Nat Commun Article Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. Nature Publishing Group UK 2017-09-21 /pmc/articles/PMC5608754/ /pubmed/28935898 http://dx.doi.org/10.1038/s41467-017-00799-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Lodish, Harvey Dougan, Stephanie K. Ploegh, Hidde L. PD-L1 is an activation-independent marker of brown adipocytes |
title | PD-L1 is an activation-independent marker of brown adipocytes |
title_full | PD-L1 is an activation-independent marker of brown adipocytes |
title_fullStr | PD-L1 is an activation-independent marker of brown adipocytes |
title_full_unstemmed | PD-L1 is an activation-independent marker of brown adipocytes |
title_short | PD-L1 is an activation-independent marker of brown adipocytes |
title_sort | pd-l1 is an activation-independent marker of brown adipocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608754/ https://www.ncbi.nlm.nih.gov/pubmed/28935898 http://dx.doi.org/10.1038/s41467-017-00799-8 |
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