Cargando…

PD-L1 is an activation-independent marker of brown adipocytes

Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-...

Descripción completa

Detalles Bibliográficos
Autores principales: Ingram, Jessica R., Dougan, Michael, Rashidian, Mohammad, Knoll, Marko, Keliher, Edmund J., Garrett, Sarah, Garforth, Scott, Blomberg, Olga S., Espinosa, Camilo, Bhan, Atul, Almo, Steven C., Weissleder, Ralph, Lodish, Harvey, Dougan, Stephanie K., Ploegh, Hidde L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608754/
https://www.ncbi.nlm.nih.gov/pubmed/28935898
http://dx.doi.org/10.1038/s41467-017-00799-8
_version_ 1783265487290892288
author Ingram, Jessica R.
Dougan, Michael
Rashidian, Mohammad
Knoll, Marko
Keliher, Edmund J.
Garrett, Sarah
Garforth, Scott
Blomberg, Olga S.
Espinosa, Camilo
Bhan, Atul
Almo, Steven C.
Weissleder, Ralph
Lodish, Harvey
Dougan, Stephanie K.
Ploegh, Hidde L.
author_facet Ingram, Jessica R.
Dougan, Michael
Rashidian, Mohammad
Knoll, Marko
Keliher, Edmund J.
Garrett, Sarah
Garforth, Scott
Blomberg, Olga S.
Espinosa, Camilo
Bhan, Atul
Almo, Steven C.
Weissleder, Ralph
Lodish, Harvey
Dougan, Stephanie K.
Ploegh, Hidde L.
author_sort Ingram, Jessica R.
collection PubMed
description Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.
format Online
Article
Text
id pubmed-5608754
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-56087542017-09-25 PD-L1 is an activation-independent marker of brown adipocytes Ingram, Jessica R. Dougan, Michael Rashidian, Mohammad Knoll, Marko Keliher, Edmund J. Garrett, Sarah Garforth, Scott Blomberg, Olga S. Espinosa, Camilo Bhan, Atul Almo, Steven C. Weissleder, Ralph Lodish, Harvey Dougan, Stephanie K. Ploegh, Hidde L. Nat Commun Article Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. Nature Publishing Group UK 2017-09-21 /pmc/articles/PMC5608754/ /pubmed/28935898 http://dx.doi.org/10.1038/s41467-017-00799-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ingram, Jessica R.
Dougan, Michael
Rashidian, Mohammad
Knoll, Marko
Keliher, Edmund J.
Garrett, Sarah
Garforth, Scott
Blomberg, Olga S.
Espinosa, Camilo
Bhan, Atul
Almo, Steven C.
Weissleder, Ralph
Lodish, Harvey
Dougan, Stephanie K.
Ploegh, Hidde L.
PD-L1 is an activation-independent marker of brown adipocytes
title PD-L1 is an activation-independent marker of brown adipocytes
title_full PD-L1 is an activation-independent marker of brown adipocytes
title_fullStr PD-L1 is an activation-independent marker of brown adipocytes
title_full_unstemmed PD-L1 is an activation-independent marker of brown adipocytes
title_short PD-L1 is an activation-independent marker of brown adipocytes
title_sort pd-l1 is an activation-independent marker of brown adipocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608754/
https://www.ncbi.nlm.nih.gov/pubmed/28935898
http://dx.doi.org/10.1038/s41467-017-00799-8
work_keys_str_mv AT ingramjessicar pdl1isanactivationindependentmarkerofbrownadipocytes
AT douganmichael pdl1isanactivationindependentmarkerofbrownadipocytes
AT rashidianmohammad pdl1isanactivationindependentmarkerofbrownadipocytes
AT knollmarko pdl1isanactivationindependentmarkerofbrownadipocytes
AT keliheredmundj pdl1isanactivationindependentmarkerofbrownadipocytes
AT garrettsarah pdl1isanactivationindependentmarkerofbrownadipocytes
AT garforthscott pdl1isanactivationindependentmarkerofbrownadipocytes
AT blombergolgas pdl1isanactivationindependentmarkerofbrownadipocytes
AT espinosacamilo pdl1isanactivationindependentmarkerofbrownadipocytes
AT bhanatul pdl1isanactivationindependentmarkerofbrownadipocytes
AT almostevenc pdl1isanactivationindependentmarkerofbrownadipocytes
AT weisslederralph pdl1isanactivationindependentmarkerofbrownadipocytes
AT lodishharvey pdl1isanactivationindependentmarkerofbrownadipocytes
AT douganstephaniek pdl1isanactivationindependentmarkerofbrownadipocytes
AT ploeghhiddel pdl1isanactivationindependentmarkerofbrownadipocytes