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Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis
Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608760/ https://www.ncbi.nlm.nih.gov/pubmed/28935952 http://dx.doi.org/10.1038/s41467-017-00698-y |
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author | Liu, Yan Conboy, Michael J. Mehdipour, Melod Liu, Yutong Tran, Thanhtra P. Blotnick, Aaron Rajan, Prasanna Santos, Thalie Cavalcante Conboy, Irina M. |
author_facet | Liu, Yan Conboy, Michael J. Mehdipour, Melod Liu, Yutong Tran, Thanhtra P. Blotnick, Aaron Rajan, Prasanna Santos, Thalie Cavalcante Conboy, Irina M. |
author_sort | Liu, Yan |
collection | PubMed |
description | Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in transplanted myoblasts, but not in host tissue, and proteins exclusive to one young mouse and transferred during parabiosis to its old partner. We use a transgenic mouse strain that ubiquitously expresses a modified tRNA methionine synthase, metRS, which preferentially incorporates the methionine surrogate azido-nor-leucine (ANL) into newly generated proteins. Using click chemistry and a modified antibody array to detect ANL-labeled proteins, we identify several ‘young’ systemic factors in old regenerating muscle of the heterochronic parabiotic partners. Our approach enables the selective profiling of mammalian proteomes in mixed biological environments such as cell and tissue transplantation, apheresis or parabiosis. |
format | Online Article Text |
id | pubmed-5608760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56087602017-09-25 Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis Liu, Yan Conboy, Michael J. Mehdipour, Melod Liu, Yutong Tran, Thanhtra P. Blotnick, Aaron Rajan, Prasanna Santos, Thalie Cavalcante Conboy, Irina M. Nat Commun Article Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in transplanted myoblasts, but not in host tissue, and proteins exclusive to one young mouse and transferred during parabiosis to its old partner. We use a transgenic mouse strain that ubiquitously expresses a modified tRNA methionine synthase, metRS, which preferentially incorporates the methionine surrogate azido-nor-leucine (ANL) into newly generated proteins. Using click chemistry and a modified antibody array to detect ANL-labeled proteins, we identify several ‘young’ systemic factors in old regenerating muscle of the heterochronic parabiotic partners. Our approach enables the selective profiling of mammalian proteomes in mixed biological environments such as cell and tissue transplantation, apheresis or parabiosis. Nature Publishing Group UK 2017-09-21 /pmc/articles/PMC5608760/ /pubmed/28935952 http://dx.doi.org/10.1038/s41467-017-00698-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Yan Conboy, Michael J. Mehdipour, Melod Liu, Yutong Tran, Thanhtra P. Blotnick, Aaron Rajan, Prasanna Santos, Thalie Cavalcante Conboy, Irina M. Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title_full | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title_fullStr | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title_full_unstemmed | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title_short | Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
title_sort | application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608760/ https://www.ncbi.nlm.nih.gov/pubmed/28935952 http://dx.doi.org/10.1038/s41467-017-00698-y |
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