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Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues
Advances in imaging have made it increasingly common to study soft tissues without first embedding them in plastic or paraffin and without using labels or stains. The process, however, usually still involves fixation and cryosectioning, which could deform the tissues. Our goal was to quantify the mo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608899/ https://www.ncbi.nlm.nih.gov/pubmed/28935889 http://dx.doi.org/10.1038/s41598-017-12006-1 |
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author | Tran, Huong Jan, Ning-Jiun Hu, Danielle Voorhees, Andrew Schuman, Joel S. Smith, Matthew A. Wollstein, Gadi Sigal, Ian A. |
author_facet | Tran, Huong Jan, Ning-Jiun Hu, Danielle Voorhees, Andrew Schuman, Joel S. Smith, Matthew A. Wollstein, Gadi Sigal, Ian A. |
author_sort | Tran, Huong |
collection | PubMed |
description | Advances in imaging have made it increasingly common to study soft tissues without first embedding them in plastic or paraffin and without using labels or stains. The process, however, usually still involves fixation and cryosectioning, which could deform the tissues. Our goal was to quantify the morphological changes of ocular tissues caused by formalin fixation and cryosectioning. From each of 6 porcine eyes, 4 regions were obtained: cornea, equatorial and posterior sclera, and posterior pole containing the optic nerve head. Samples were imaged using visible light microscopy fresh, 1-minute and 24-hours post-fixation, and post-cryosectioning. Effects were assessed by 14 parameters representing sample size and shape. Overall, formalin fixation and sectioning caused only minimal changes to the ocular tissues, with average percentage parameter differences of 0.1%, 1%, and 1.2% between fresh and post-fixing by 1 minute, 24 hours, and post-cryosectioning, respectively. Parameter changes were not directional, and were only weakly dependent on the duration of fixation and the region of the eye. These results demonstrate that formalin fixation and cryosectioning are good choices for studying ocular tissue morphology and structure, as they do not cause the large tissue shrinkage or distortions typically associated with other, more complicated, techniques. |
format | Online Article Text |
id | pubmed-5608899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56088992017-10-10 Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues Tran, Huong Jan, Ning-Jiun Hu, Danielle Voorhees, Andrew Schuman, Joel S. Smith, Matthew A. Wollstein, Gadi Sigal, Ian A. Sci Rep Article Advances in imaging have made it increasingly common to study soft tissues without first embedding them in plastic or paraffin and without using labels or stains. The process, however, usually still involves fixation and cryosectioning, which could deform the tissues. Our goal was to quantify the morphological changes of ocular tissues caused by formalin fixation and cryosectioning. From each of 6 porcine eyes, 4 regions were obtained: cornea, equatorial and posterior sclera, and posterior pole containing the optic nerve head. Samples were imaged using visible light microscopy fresh, 1-minute and 24-hours post-fixation, and post-cryosectioning. Effects were assessed by 14 parameters representing sample size and shape. Overall, formalin fixation and sectioning caused only minimal changes to the ocular tissues, with average percentage parameter differences of 0.1%, 1%, and 1.2% between fresh and post-fixing by 1 minute, 24 hours, and post-cryosectioning, respectively. Parameter changes were not directional, and were only weakly dependent on the duration of fixation and the region of the eye. These results demonstrate that formalin fixation and cryosectioning are good choices for studying ocular tissue morphology and structure, as they do not cause the large tissue shrinkage or distortions typically associated with other, more complicated, techniques. Nature Publishing Group UK 2017-09-21 /pmc/articles/PMC5608899/ /pubmed/28935889 http://dx.doi.org/10.1038/s41598-017-12006-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tran, Huong Jan, Ning-Jiun Hu, Danielle Voorhees, Andrew Schuman, Joel S. Smith, Matthew A. Wollstein, Gadi Sigal, Ian A. Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title | Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title_full | Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title_fullStr | Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title_full_unstemmed | Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title_short | Formalin Fixation and Cryosectioning Cause Only Minimal Changes in Shape or Size of Ocular Tissues |
title_sort | formalin fixation and cryosectioning cause only minimal changes in shape or size of ocular tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5608899/ https://www.ncbi.nlm.nih.gov/pubmed/28935889 http://dx.doi.org/10.1038/s41598-017-12006-1 |
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