Cargando…

GFAP expression in injured astrocytes in rats

Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary c...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Shuquan, Wu, Minfei, Peng, Chuangang, Zhao, Guanjie, Gu, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609138/
https://www.ncbi.nlm.nih.gov/pubmed/28962102
http://dx.doi.org/10.3892/etm.2017.4760
_version_ 1783265558230204416
author Zhang, Shuquan
Wu, Minfei
Peng, Chuangang
Zhao, Guanjie
Gu, Rui
author_facet Zhang, Shuquan
Wu, Minfei
Peng, Chuangang
Zhao, Guanjie
Gu, Rui
author_sort Zhang, Shuquan
collection PubMed
description Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3ε by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3ε protein localization by double-labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3ε expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries.
format Online
Article
Text
id pubmed-5609138
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-56091382017-09-28 GFAP expression in injured astrocytes in rats Zhang, Shuquan Wu, Minfei Peng, Chuangang Zhao, Guanjie Gu, Rui Exp Ther Med Articles Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3ε by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3ε protein localization by double-labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3ε expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries. D.A. Spandidos 2017-09 2017-07-10 /pmc/articles/PMC5609138/ /pubmed/28962102 http://dx.doi.org/10.3892/etm.2017.4760 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Shuquan
Wu, Minfei
Peng, Chuangang
Zhao, Guanjie
Gu, Rui
GFAP expression in injured astrocytes in rats
title GFAP expression in injured astrocytes in rats
title_full GFAP expression in injured astrocytes in rats
title_fullStr GFAP expression in injured astrocytes in rats
title_full_unstemmed GFAP expression in injured astrocytes in rats
title_short GFAP expression in injured astrocytes in rats
title_sort gfap expression in injured astrocytes in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609138/
https://www.ncbi.nlm.nih.gov/pubmed/28962102
http://dx.doi.org/10.3892/etm.2017.4760
work_keys_str_mv AT zhangshuquan gfapexpressionininjuredastrocytesinrats
AT wuminfei gfapexpressionininjuredastrocytesinrats
AT pengchuangang gfapexpressionininjuredastrocytesinrats
AT zhaoguanjie gfapexpressionininjuredastrocytesinrats
AT gurui gfapexpressionininjuredastrocytesinrats