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Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly

The aim of the study was to examine the correlation between KCNJ11 gene polymorphism and metabolic syndrome in elderly patients. From January 2014 to January 2015, 54 elderly patients with metabolic syndrome were enrolled in this study as the observation group. During the same period, 46 healthy eld...

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Autores principales: Jiang, Fan, Liu, Ning, Chen, Xiao Zhuang, Han, Kun Yuan, Zhu, Cai Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609148/
https://www.ncbi.nlm.nih.gov/pubmed/28962121
http://dx.doi.org/10.3892/etm.2017.4714
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author Jiang, Fan
Liu, Ning
Chen, Xiao Zhuang
Han, Kun Yuan
Zhu, Cai Zhong
author_facet Jiang, Fan
Liu, Ning
Chen, Xiao Zhuang
Han, Kun Yuan
Zhu, Cai Zhong
author_sort Jiang, Fan
collection PubMed
description The aim of the study was to examine the correlation between KCNJ11 gene polymorphism and metabolic syndrome in elderly patients. From January 2014 to January 2015, 54 elderly patients with metabolic syndrome were enrolled in this study as the observation group. During the same period, 46 healthy elderly individuals were enrolled in this study as the control group. KCNJ11 gene polymorphism (rs28502) was analyzed using polymerase chain reaction-restriction fragment length polymorphism. The expression levels of mRNA in different genotypes were detected using FQ-PCR. ELISA was used to evaluate the KCNJ11 protein expression in different genotypes. KCNJ11 gene polymorphism and metabolic syndrome was studied by measuring the blood pressure levels in patients with different genotypes. Three genotypes of KCNJ11 gene in rs28502 were CC, CT and TT. The CC, CT and TT genotype frequencies in healthy population were 8.5, 9.2 and 82.2%, respectively, while the genotype frequencies in patients with metabolic syndrome were 42.4, 49.8 and 7.8%, respectively. There were significant differences between groups (P≤0.05). However, the genotype frequencies of C/T in healthy individuals and metabolic syndrome patients were 35.3 and 38.3%, respectively. There were no significant differences between groups (P>0.05). FQ-PCR results showed that the KCNJ11 mRNA expression levels in the control and observation groups had no significant differences (P>0.05). However, the results obtained from ELISA analysis revealed that KCNJ11 protein expression level in the observation group was significantly higher than that in the control group (P<0.05). In conclusion, KCNJ11 gene polymorphism is associated with metabolic syndrome in the elderly. Elderly patients with the CC and TT genotypes are more likely to develop metabolic syndrome.
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spelling pubmed-56091482017-09-28 Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly Jiang, Fan Liu, Ning Chen, Xiao Zhuang Han, Kun Yuan Zhu, Cai Zhong Exp Ther Med Articles The aim of the study was to examine the correlation between KCNJ11 gene polymorphism and metabolic syndrome in elderly patients. From January 2014 to January 2015, 54 elderly patients with metabolic syndrome were enrolled in this study as the observation group. During the same period, 46 healthy elderly individuals were enrolled in this study as the control group. KCNJ11 gene polymorphism (rs28502) was analyzed using polymerase chain reaction-restriction fragment length polymorphism. The expression levels of mRNA in different genotypes were detected using FQ-PCR. ELISA was used to evaluate the KCNJ11 protein expression in different genotypes. KCNJ11 gene polymorphism and metabolic syndrome was studied by measuring the blood pressure levels in patients with different genotypes. Three genotypes of KCNJ11 gene in rs28502 were CC, CT and TT. The CC, CT and TT genotype frequencies in healthy population were 8.5, 9.2 and 82.2%, respectively, while the genotype frequencies in patients with metabolic syndrome were 42.4, 49.8 and 7.8%, respectively. There were significant differences between groups (P≤0.05). However, the genotype frequencies of C/T in healthy individuals and metabolic syndrome patients were 35.3 and 38.3%, respectively. There were no significant differences between groups (P>0.05). FQ-PCR results showed that the KCNJ11 mRNA expression levels in the control and observation groups had no significant differences (P>0.05). However, the results obtained from ELISA analysis revealed that KCNJ11 protein expression level in the observation group was significantly higher than that in the control group (P<0.05). In conclusion, KCNJ11 gene polymorphism is associated with metabolic syndrome in the elderly. Elderly patients with the CC and TT genotypes are more likely to develop metabolic syndrome. D.A. Spandidos 2017-09 2017-06-30 /pmc/articles/PMC5609148/ /pubmed/28962121 http://dx.doi.org/10.3892/etm.2017.4714 Text en Copyright: © Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Jiang, Fan
Liu, Ning
Chen, Xiao Zhuang
Han, Kun Yuan
Zhu, Cai Zhong
Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title_full Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title_fullStr Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title_full_unstemmed Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title_short Study on the correlation between KCNJ11 gene polymorphism and metabolic syndrome in the elderly
title_sort study on the correlation between kcnj11 gene polymorphism and metabolic syndrome in the elderly
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609148/
https://www.ncbi.nlm.nih.gov/pubmed/28962121
http://dx.doi.org/10.3892/etm.2017.4714
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