Cargando…

Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system

Shen-Zhi-Ling (SZL) oral liquid is a traditional Chinese medicine formula that is mainly used for the clinical treatment of mild to moderate Alzheimer's disease (AD). The aim of the present study was to investigate the effects and underlying mechanisms of SZL treatment on AD. APP/PS1 transgenic...

Descripción completa

Detalles Bibliográficos
Autores principales: Xing, Sanli, Shen, Dingzhu, Chen, Chuan, Wu, Beiling, Chi, Huiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609159/
https://www.ncbi.nlm.nih.gov/pubmed/28962110
http://dx.doi.org/10.3892/etm.2017.4732
_version_ 1783265561989349376
author Xing, Sanli
Shen, Dingzhu
Chen, Chuan
Wu, Beiling
Chi, Huiying
author_facet Xing, Sanli
Shen, Dingzhu
Chen, Chuan
Wu, Beiling
Chi, Huiying
author_sort Xing, Sanli
collection PubMed
description Shen-Zhi-Ling (SZL) oral liquid is a traditional Chinese medicine formula that is mainly used for the clinical treatment of mild to moderate Alzheimer's disease (AD). The aim of the present study was to investigate the effects and underlying mechanisms of SZL treatment on AD. APP/PS1 transgenic mice were utilized to evaluate the effect of SZL treatment (0.5 g/20 g/day). Morris water maze and Thioflavin S staining analyses were used to evaluate the cognitive impairment and β-amyloid plaques, respectively, while quantitative polymerase chain reaction and western blot analysis were performed to examine the mRNA and protein expression levels of heme oxygenase 1 (HO-1) and biliverdin reductase (BVR). Furthermore, immunofluorescence staining was used to measure the BVR and HO-1 protein levels in the hippocampus. The findings of the current study demonstrated that SZL treatment was able to ameliorate the impairment of memory and reduce the accumulation of amyloid plaques, and its ameliorating effects may be attributed to the modulation of the HO-1/BVR system in the hippocampus. These results indicate that SZL may be a possible complementary and alternative therapy to delay the development of AD.
format Online
Article
Text
id pubmed-5609159
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-56091592017-09-28 Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system Xing, Sanli Shen, Dingzhu Chen, Chuan Wu, Beiling Chi, Huiying Exp Ther Med Articles Shen-Zhi-Ling (SZL) oral liquid is a traditional Chinese medicine formula that is mainly used for the clinical treatment of mild to moderate Alzheimer's disease (AD). The aim of the present study was to investigate the effects and underlying mechanisms of SZL treatment on AD. APP/PS1 transgenic mice were utilized to evaluate the effect of SZL treatment (0.5 g/20 g/day). Morris water maze and Thioflavin S staining analyses were used to evaluate the cognitive impairment and β-amyloid plaques, respectively, while quantitative polymerase chain reaction and western blot analysis were performed to examine the mRNA and protein expression levels of heme oxygenase 1 (HO-1) and biliverdin reductase (BVR). Furthermore, immunofluorescence staining was used to measure the BVR and HO-1 protein levels in the hippocampus. The findings of the current study demonstrated that SZL treatment was able to ameliorate the impairment of memory and reduce the accumulation of amyloid plaques, and its ameliorating effects may be attributed to the modulation of the HO-1/BVR system in the hippocampus. These results indicate that SZL may be a possible complementary and alternative therapy to delay the development of AD. D.A. Spandidos 2017-09 2017-07-09 /pmc/articles/PMC5609159/ /pubmed/28962110 http://dx.doi.org/10.3892/etm.2017.4732 Text en Copyright: © Xing et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xing, Sanli
Shen, Dingzhu
Chen, Chuan
Wu, Beiling
Chi, Huiying
Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title_full Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title_fullStr Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title_full_unstemmed Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title_short Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
title_sort effect of the herbal formulation shen-zhi-ling on an app/ps1 mouse model of alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609159/
https://www.ncbi.nlm.nih.gov/pubmed/28962110
http://dx.doi.org/10.3892/etm.2017.4732
work_keys_str_mv AT xingsanli effectoftheherbalformulationshenzhilingonanappps1mousemodelofalzheimersdiseasebymodulatingthebiliverdinreductasehemeoxygenase1system
AT shendingzhu effectoftheherbalformulationshenzhilingonanappps1mousemodelofalzheimersdiseasebymodulatingthebiliverdinreductasehemeoxygenase1system
AT chenchuan effectoftheherbalformulationshenzhilingonanappps1mousemodelofalzheimersdiseasebymodulatingthebiliverdinreductasehemeoxygenase1system
AT wubeiling effectoftheherbalformulationshenzhilingonanappps1mousemodelofalzheimersdiseasebymodulatingthebiliverdinreductasehemeoxygenase1system
AT chihuiying effectoftheherbalformulationshenzhilingonanappps1mousemodelofalzheimersdiseasebymodulatingthebiliverdinreductasehemeoxygenase1system