Cargando…
Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance
The present study determined the changes in the expression levels of MYPT1, CPI-17 and MLC20 in the ileum of mice with neonatal induced necrotizing enterocolitis (NEC) to provide a basis for a pathogenesis model that includes smooth muscle changes during NEC. A group of 7-day-old BALB/c mice were fe...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609160/ https://www.ncbi.nlm.nih.gov/pubmed/28962146 http://dx.doi.org/10.3892/etm.2017.4783 |
_version_ | 1783265562239959040 |
---|---|
author | Yin, Yinyu Li, Yiping Pan, Jian Tang, Ruze Zhu, Jie Qin, Zhenfang Xu, Xiaobing Wang, Jian |
author_facet | Yin, Yinyu Li, Yiping Pan, Jian Tang, Ruze Zhu, Jie Qin, Zhenfang Xu, Xiaobing Wang, Jian |
author_sort | Yin, Yinyu |
collection | PubMed |
description | The present study determined the changes in the expression levels of MYPT1, CPI-17 and MLC20 in the ileum of mice with neonatal induced necrotizing enterocolitis (NEC) to provide a basis for a pathogenesis model that includes smooth muscle changes during NEC. A group of 7-day-old BALB/c mice were fed with formula (40 µl/g, 5 times/day) and given hypoxia treatments (5% O(2) and 95% N(2) for 10 min, twice daily) for 4 days to induce NEC and establish a mouse model. A control group of 7-day-old BALB/c mice were left with their mother for the duration of the treatment. After establishing the model, the two groups of mice were sacrificed, and the terminal ileum tissue was collected and subjected to western blot analysis and immunohistochemistry. The results showed the expression levels of MYPT1 and pMYPT1 in the ileum of the mice in the NEC group were lower than those in the control group (P<0.01). The levels of CPI17 and pCPI17 were higher in the NEC group compared with those in the control group. The expression level of MLC20 in NEC group was lower than that in the control group (P<0.01), but the level of pMLC20 in the NEC group was higher (P<0.05). The results of immunohistochemistry showed that the staining intensities of MYPT1, CPI-17 and MLC20 in the NEC group were lighter than those in the control group, and the proportion of positive cells was also lower in the NEC group (P<0.01). Taken together our results suggest that establishment of NEC is accompanied by changes in the protein levels of MYPT1 and pCPI-17, which can regulate smooth muscle contraction in the ileum. |
format | Online Article Text |
id | pubmed-5609160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-56091602017-09-28 Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance Yin, Yinyu Li, Yiping Pan, Jian Tang, Ruze Zhu, Jie Qin, Zhenfang Xu, Xiaobing Wang, Jian Exp Ther Med Articles The present study determined the changes in the expression levels of MYPT1, CPI-17 and MLC20 in the ileum of mice with neonatal induced necrotizing enterocolitis (NEC) to provide a basis for a pathogenesis model that includes smooth muscle changes during NEC. A group of 7-day-old BALB/c mice were fed with formula (40 µl/g, 5 times/day) and given hypoxia treatments (5% O(2) and 95% N(2) for 10 min, twice daily) for 4 days to induce NEC and establish a mouse model. A control group of 7-day-old BALB/c mice were left with their mother for the duration of the treatment. After establishing the model, the two groups of mice were sacrificed, and the terminal ileum tissue was collected and subjected to western blot analysis and immunohistochemistry. The results showed the expression levels of MYPT1 and pMYPT1 in the ileum of the mice in the NEC group were lower than those in the control group (P<0.01). The levels of CPI17 and pCPI17 were higher in the NEC group compared with those in the control group. The expression level of MLC20 in NEC group was lower than that in the control group (P<0.01), but the level of pMLC20 in the NEC group was higher (P<0.05). The results of immunohistochemistry showed that the staining intensities of MYPT1, CPI-17 and MLC20 in the NEC group were lighter than those in the control group, and the proportion of positive cells was also lower in the NEC group (P<0.01). Taken together our results suggest that establishment of NEC is accompanied by changes in the protein levels of MYPT1 and pCPI-17, which can regulate smooth muscle contraction in the ileum. D.A. Spandidos 2017-09 2017-07-12 /pmc/articles/PMC5609160/ /pubmed/28962146 http://dx.doi.org/10.3892/etm.2017.4783 Text en Copyright: © Yin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yin, Yinyu Li, Yiping Pan, Jian Tang, Ruze Zhu, Jie Qin, Zhenfang Xu, Xiaobing Wang, Jian Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title | Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title_full | Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title_fullStr | Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title_full_unstemmed | Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title_short | Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance |
title_sort | expression of mypt1, cpi-17 and mlc20 in ileum of neonatal mouse nec model and its significance |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609160/ https://www.ncbi.nlm.nih.gov/pubmed/28962146 http://dx.doi.org/10.3892/etm.2017.4783 |
work_keys_str_mv | AT yinyinyu expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT liyiping expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT panjian expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT tangruze expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT zhujie expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT qinzhenfang expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT xuxiaobing expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance AT wangjian expressionofmypt1cpi17andmlc20inileumofneonatalmousenecmodelanditssignificance |