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Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections

Polypropylene mesh implants are routinely used to repair abdominal wall defects or incisional hernia. However, complications associated with mesh implantation, such as mesh-related infections, can cause serious problems and may require complete surgical removal. Hence, the aim of the present study w...

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Autores principales: Reinbold, Jochen, Hierlemann, Teresa, Urich, Lukas, Uhde, Ann-Kristin, Müller, Ingrid, Weindl, Tobias, Vogel, Ulrich, Schlensak, Christian, Wendel, Hans Peter, Krajewski, Stefanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609798/
https://www.ncbi.nlm.nih.gov/pubmed/29075100
http://dx.doi.org/10.2147/DDDT.S138510
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author Reinbold, Jochen
Hierlemann, Teresa
Urich, Lukas
Uhde, Ann-Kristin
Müller, Ingrid
Weindl, Tobias
Vogel, Ulrich
Schlensak, Christian
Wendel, Hans Peter
Krajewski, Stefanie
author_facet Reinbold, Jochen
Hierlemann, Teresa
Urich, Lukas
Uhde, Ann-Kristin
Müller, Ingrid
Weindl, Tobias
Vogel, Ulrich
Schlensak, Christian
Wendel, Hans Peter
Krajewski, Stefanie
author_sort Reinbold, Jochen
collection PubMed
description Polypropylene mesh implants are routinely used to repair abdominal wall defects or incisional hernia. However, complications associated with mesh implantation, such as mesh-related infections, can cause serious problems and may require complete surgical removal. Hence, the aim of the present study was the development of a safe and efficient coating to reduce postoperative mesh infections. Biodegradable poly(lactide-co-glycolide acid) microspheres loaded with rifampicin as an antibacterial agent were prepared through single emulsion evaporation method. The particle size distribution (67.93±3.39 μm for rifampicin-loaded microspheres and 64.43±3.61 μm for unloaded microspheres) was measured by laser diffraction. Furthermore, the encapsulation efficiency of rifampicin (61.5%±2.58%) was detected via ultraviolet–visible (UV/Vis) spectroscopy. The drug release of rifampicin-loaded microspheres was detected by UV/Vis spectroscopy over a period of 60 days. After 60 days, 92.40%±3.54% of the encapsulated rifampicin has been continuously released. The viability of BJ fibroblasts after incubation with unloaded and rifampicin-loaded microspheres was investigated using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, which showed no adverse effects on the cells. Furthermore, the antibacterial impact of rifampicin-loaded microspheres and mesh implants, coated with the antibacterial microspheres, was investigated using an agar diffusion model with Staphylococcus aureus. The coated mesh implants were also tested in an in vivo mouse model of staphylococcal infection and resulted in a 100% protection against mesh implant infections or biofilm formation shown by macroscopic imaging, scanning electron microscopy, and histological examinations. This effective antibacterial mesh coating combining the benefit of a controlled drug delivery system and a potent antibacterial agent possesses the ability to significantly reduce postoperative implant infections.
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spelling pubmed-56097982017-10-26 Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections Reinbold, Jochen Hierlemann, Teresa Urich, Lukas Uhde, Ann-Kristin Müller, Ingrid Weindl, Tobias Vogel, Ulrich Schlensak, Christian Wendel, Hans Peter Krajewski, Stefanie Drug Des Devel Ther Original Research Polypropylene mesh implants are routinely used to repair abdominal wall defects or incisional hernia. However, complications associated with mesh implantation, such as mesh-related infections, can cause serious problems and may require complete surgical removal. Hence, the aim of the present study was the development of a safe and efficient coating to reduce postoperative mesh infections. Biodegradable poly(lactide-co-glycolide acid) microspheres loaded with rifampicin as an antibacterial agent were prepared through single emulsion evaporation method. The particle size distribution (67.93±3.39 μm for rifampicin-loaded microspheres and 64.43±3.61 μm for unloaded microspheres) was measured by laser diffraction. Furthermore, the encapsulation efficiency of rifampicin (61.5%±2.58%) was detected via ultraviolet–visible (UV/Vis) spectroscopy. The drug release of rifampicin-loaded microspheres was detected by UV/Vis spectroscopy over a period of 60 days. After 60 days, 92.40%±3.54% of the encapsulated rifampicin has been continuously released. The viability of BJ fibroblasts after incubation with unloaded and rifampicin-loaded microspheres was investigated using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, which showed no adverse effects on the cells. Furthermore, the antibacterial impact of rifampicin-loaded microspheres and mesh implants, coated with the antibacterial microspheres, was investigated using an agar diffusion model with Staphylococcus aureus. The coated mesh implants were also tested in an in vivo mouse model of staphylococcal infection and resulted in a 100% protection against mesh implant infections or biofilm formation shown by macroscopic imaging, scanning electron microscopy, and histological examinations. This effective antibacterial mesh coating combining the benefit of a controlled drug delivery system and a potent antibacterial agent possesses the ability to significantly reduce postoperative implant infections. Dove Medical Press 2017-09-18 /pmc/articles/PMC5609798/ /pubmed/29075100 http://dx.doi.org/10.2147/DDDT.S138510 Text en © 2017 Reinbold et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Reinbold, Jochen
Hierlemann, Teresa
Urich, Lukas
Uhde, Ann-Kristin
Müller, Ingrid
Weindl, Tobias
Vogel, Ulrich
Schlensak, Christian
Wendel, Hans Peter
Krajewski, Stefanie
Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title_full Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title_fullStr Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title_full_unstemmed Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title_short Biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
title_sort biodegradable rifampicin-releasing coating of surgical meshes for the prevention of bacterial infections
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609798/
https://www.ncbi.nlm.nih.gov/pubmed/29075100
http://dx.doi.org/10.2147/DDDT.S138510
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