Cargando…
Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach
Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609834/ https://www.ncbi.nlm.nih.gov/pubmed/28948217 http://dx.doi.org/10.1126/sciadv.1603187 |
_version_ | 1783265673951051776 |
---|---|
author | Shevchenko, Vitaly Mager, Thomas Kovalev, Kirill Polovinkin, Vitaly Alekseev, Alexey Juettner, Josephine Chizhov, Igor Bamann, Christian Vavourakis, Charlotte Ghai, Rohit Gushchin, Ivan Borshchevskiy, Valentin Rogachev, Andrey Melnikov, Igor Popov, Alexander Balandin, Taras Rodriguez-Valera, Francisco Manstein, Dietmar J. Bueldt, Georg Bamberg, Ernst Gordeliy, Valentin |
author_facet | Shevchenko, Vitaly Mager, Thomas Kovalev, Kirill Polovinkin, Vitaly Alekseev, Alexey Juettner, Josephine Chizhov, Igor Bamann, Christian Vavourakis, Charlotte Ghai, Rohit Gushchin, Ivan Borshchevskiy, Valentin Rogachev, Andrey Melnikov, Igor Popov, Alexander Balandin, Taras Rodriguez-Valera, Francisco Manstein, Dietmar J. Bueldt, Georg Bamberg, Ernst Gordeliy, Valentin |
author_sort | Shevchenko, Vitaly |
collection | PubMed |
description | Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, Escherichia coli cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon Nanosalina (NsXeR) and suggest a mechanism of inward proton pumping. We demonstrate that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins. |
format | Online Article Text |
id | pubmed-5609834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56098342017-09-25 Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach Shevchenko, Vitaly Mager, Thomas Kovalev, Kirill Polovinkin, Vitaly Alekseev, Alexey Juettner, Josephine Chizhov, Igor Bamann, Christian Vavourakis, Charlotte Ghai, Rohit Gushchin, Ivan Borshchevskiy, Valentin Rogachev, Andrey Melnikov, Igor Popov, Alexander Balandin, Taras Rodriguez-Valera, Francisco Manstein, Dietmar J. Bueldt, Georg Bamberg, Ernst Gordeliy, Valentin Sci Adv Research Articles Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, Escherichia coli cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon Nanosalina (NsXeR) and suggest a mechanism of inward proton pumping. We demonstrate that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins. American Association for the Advancement of Science 2017-09-22 /pmc/articles/PMC5609834/ /pubmed/28948217 http://dx.doi.org/10.1126/sciadv.1603187 Text en Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Shevchenko, Vitaly Mager, Thomas Kovalev, Kirill Polovinkin, Vitaly Alekseev, Alexey Juettner, Josephine Chizhov, Igor Bamann, Christian Vavourakis, Charlotte Ghai, Rohit Gushchin, Ivan Borshchevskiy, Valentin Rogachev, Andrey Melnikov, Igor Popov, Alexander Balandin, Taras Rodriguez-Valera, Francisco Manstein, Dietmar J. Bueldt, Georg Bamberg, Ernst Gordeliy, Valentin Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title | Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title_full | Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title_fullStr | Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title_full_unstemmed | Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title_short | Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach |
title_sort | inward h(+) pump xenorhodopsin: mechanism and alternative optogenetic approach |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609834/ https://www.ncbi.nlm.nih.gov/pubmed/28948217 http://dx.doi.org/10.1126/sciadv.1603187 |
work_keys_str_mv | AT shevchenkovitaly inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT magerthomas inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT kovalevkirill inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT polovinkinvitaly inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT alekseevalexey inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT juettnerjosephine inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT chizhovigor inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT bamannchristian inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT vavourakischarlotte inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT ghairohit inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT gushchinivan inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT borshchevskiyvalentin inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT rogachevandrey inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT melnikovigor inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT popovalexander inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT balandintaras inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT rodriguezvalerafrancisco inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT mansteindietmarj inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT bueldtgeorg inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT bambergernst inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach AT gordeliyvalentin inwardhpumpxenorhodopsinmechanismandalternativeoptogeneticapproach |