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MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6
Aberrantly expressed microRNAs contribute to the initiation and progression of human cancer. MiRNA-187 has been reported in nasopharyngeal, renal, pancreatic, prostate, and esophageal cancer, and acts as a tumor suppressor or oncogene. However, the underlying function of miRNA-187 in cervical cancer...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609891/ https://www.ncbi.nlm.nih.gov/pubmed/28968959 http://dx.doi.org/10.18632/oncotarget.17516 |
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author | Lin, Mao Xue, Xiang-Yang Liang, Shu-Zhen Li, Yin-Xiong Lv, You-Yong He, Li-Hua Xu, Ke-Cheng Zhang, Li-Fang Chen, Ji-Bing Niu, Li-Zhi |
author_facet | Lin, Mao Xue, Xiang-Yang Liang, Shu-Zhen Li, Yin-Xiong Lv, You-Yong He, Li-Hua Xu, Ke-Cheng Zhang, Li-Fang Chen, Ji-Bing Niu, Li-Zhi |
author_sort | Lin, Mao |
collection | PubMed |
description | Aberrantly expressed microRNAs contribute to the initiation and progression of human cancer. MiRNA-187 has been reported in nasopharyngeal, renal, pancreatic, prostate, and esophageal cancer, and acts as a tumor suppressor or oncogene. However, the underlying function of miRNA-187 in cervical cancer remains largely unexplored. In the present study, we demonstrated significantly miRNA-187 down-regulation in cervical cancer tissues and cell lines compared to their normal counterparts. Kaplan-Meier analysis revealed that decreased miRNA-187 was closely associated with shorter overall survival and relapse-free survival. Gain- and loss-of-function studies showed that miRNA-187 suppressed cervical cancer cell proliferation, migration, and invasion, and promoted cervical cancer cell apoptosis. Furthermore, luciferase reporter assay determined that human papillomavirus 16 E6 was a direct functional target of miRNA-187. Taken together, our findings indicate the essential role of miRNA-187 in suppressing cervical cancer progression and indicate a novel link between miRNA-187 and human papillomavirus 16 E6 in cervical cancer. |
format | Online Article Text |
id | pubmed-5609891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56098912017-09-29 MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 Lin, Mao Xue, Xiang-Yang Liang, Shu-Zhen Li, Yin-Xiong Lv, You-Yong He, Li-Hua Xu, Ke-Cheng Zhang, Li-Fang Chen, Ji-Bing Niu, Li-Zhi Oncotarget Research Paper Aberrantly expressed microRNAs contribute to the initiation and progression of human cancer. MiRNA-187 has been reported in nasopharyngeal, renal, pancreatic, prostate, and esophageal cancer, and acts as a tumor suppressor or oncogene. However, the underlying function of miRNA-187 in cervical cancer remains largely unexplored. In the present study, we demonstrated significantly miRNA-187 down-regulation in cervical cancer tissues and cell lines compared to their normal counterparts. Kaplan-Meier analysis revealed that decreased miRNA-187 was closely associated with shorter overall survival and relapse-free survival. Gain- and loss-of-function studies showed that miRNA-187 suppressed cervical cancer cell proliferation, migration, and invasion, and promoted cervical cancer cell apoptosis. Furthermore, luciferase reporter assay determined that human papillomavirus 16 E6 was a direct functional target of miRNA-187. Taken together, our findings indicate the essential role of miRNA-187 in suppressing cervical cancer progression and indicate a novel link between miRNA-187 and human papillomavirus 16 E6 in cervical cancer. Impact Journals LLC 2017-04-29 /pmc/articles/PMC5609891/ /pubmed/28968959 http://dx.doi.org/10.18632/oncotarget.17516 Text en Copyright: © 2017 Lin et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Lin, Mao Xue, Xiang-Yang Liang, Shu-Zhen Li, Yin-Xiong Lv, You-Yong He, Li-Hua Xu, Ke-Cheng Zhang, Li-Fang Chen, Ji-Bing Niu, Li-Zhi MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title | MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title_full | MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title_fullStr | MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title_full_unstemmed | MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title_short | MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6 |
title_sort | mir-187 overexpression inhibits cervical cancer progression by targeting hpv16 e6 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609891/ https://www.ncbi.nlm.nih.gov/pubmed/28968959 http://dx.doi.org/10.18632/oncotarget.17516 |
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