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Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders

OBJECTIVE: Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders. Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties. The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PB...

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Autores principales: Michalak, Slawomir, Florczak-Wyspiańska, Jolanta, Rybacka-Mossakowska, Joanna, Ambrosius, Wojciech, Osztynowicz, Krystyna, Baszczuk, Aleksandra, Kozubski, Wojciech, Wysocka, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610844/
https://www.ncbi.nlm.nih.gov/pubmed/29081897
http://dx.doi.org/10.1155/2017/9703574
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author Michalak, Slawomir
Florczak-Wyspiańska, Jolanta
Rybacka-Mossakowska, Joanna
Ambrosius, Wojciech
Osztynowicz, Krystyna
Baszczuk, Aleksandra
Kozubski, Wojciech
Wysocka, Ewa
author_facet Michalak, Slawomir
Florczak-Wyspiańska, Jolanta
Rybacka-Mossakowska, Joanna
Ambrosius, Wojciech
Osztynowicz, Krystyna
Baszczuk, Aleksandra
Kozubski, Wojciech
Wysocka, Ewa
author_sort Michalak, Slawomir
collection PubMed
description OBJECTIVE: Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders. Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties. The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the SIRT3 activity in patients with movement disorders. METHODS: Mitochondrial respiration was analyzed in intact PBMCs using the ROUTINE, LEAK, electron transfer system (ETS), and residual oxygen consumption (ROX) protocol by means of high-resolution respirometry. The SIRT3 expression and PBMC activity were measured using fluorometry. Ultrasound measurements of the echogenicity of the substantia nigra and the diameter of the 3rd ventricle were also performed. RESULTS: Patients with movement disorders exhibited a lower ROUTINE respiration than controls (P = 0.0237). Reduced oxygen fluxes in the LEAK (P = 0.033) and ROX (P = 0.0486) states were observed in patients with movement disorders compared with controls. Decreased ROUTINE respiration (P = 0.007) and oxygen flux in the LEAK state (P = 0.0203) were observed in patients with PD with substantia nigra hyperechogenicity compared with controls. Decreased SIRT 3 deacetylase activity was found in patients with movement disorders. CONCLUSION: Impaired mitochondrial respiration in intact PBMCs was associated with inhibited SIRT3 activity and neurodegeneration measures evaluated using ultrasound in patients with PD.
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spelling pubmed-56108442017-10-29 Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders Michalak, Slawomir Florczak-Wyspiańska, Jolanta Rybacka-Mossakowska, Joanna Ambrosius, Wojciech Osztynowicz, Krystyna Baszczuk, Aleksandra Kozubski, Wojciech Wysocka, Ewa Oxid Med Cell Longev Research Article OBJECTIVE: Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders. Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties. The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the SIRT3 activity in patients with movement disorders. METHODS: Mitochondrial respiration was analyzed in intact PBMCs using the ROUTINE, LEAK, electron transfer system (ETS), and residual oxygen consumption (ROX) protocol by means of high-resolution respirometry. The SIRT3 expression and PBMC activity were measured using fluorometry. Ultrasound measurements of the echogenicity of the substantia nigra and the diameter of the 3rd ventricle were also performed. RESULTS: Patients with movement disorders exhibited a lower ROUTINE respiration than controls (P = 0.0237). Reduced oxygen fluxes in the LEAK (P = 0.033) and ROX (P = 0.0486) states were observed in patients with movement disorders compared with controls. Decreased ROUTINE respiration (P = 0.007) and oxygen flux in the LEAK state (P = 0.0203) were observed in patients with PD with substantia nigra hyperechogenicity compared with controls. Decreased SIRT 3 deacetylase activity was found in patients with movement disorders. CONCLUSION: Impaired mitochondrial respiration in intact PBMCs was associated with inhibited SIRT3 activity and neurodegeneration measures evaluated using ultrasound in patients with PD. Hindawi 2017 2017-09-10 /pmc/articles/PMC5610844/ /pubmed/29081897 http://dx.doi.org/10.1155/2017/9703574 Text en Copyright © 2017 Slawomir Michalak et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Michalak, Slawomir
Florczak-Wyspiańska, Jolanta
Rybacka-Mossakowska, Joanna
Ambrosius, Wojciech
Osztynowicz, Krystyna
Baszczuk, Aleksandra
Kozubski, Wojciech
Wysocka, Ewa
Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title_full Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title_fullStr Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title_full_unstemmed Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title_short Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders
title_sort mitochondrial respiration in intact peripheral blood mononuclear cells and sirtuin 3 activity in patients with movement disorders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610844/
https://www.ncbi.nlm.nih.gov/pubmed/29081897
http://dx.doi.org/10.1155/2017/9703574
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