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Coliform Bacteria for Bioremediation of Waste Hydrocarbons
Raw, domestic sewage of Kuwait City contained about 10(6) ml(−1) colony forming units of Enterobacter hormaechei subsp. oharae (56.6%), Klebsiella spp. (36%), and Escherichia coli (7.4%), as characterized by their 16S rRNA-gene sequences. The isolated coliforms grew successfully on a mineral medium...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610891/ https://www.ncbi.nlm.nih.gov/pubmed/29082238 http://dx.doi.org/10.1155/2017/1838072 |
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author | Khanafer, Majida Al-Awadhi, Husain Radwan, Samir |
author_facet | Khanafer, Majida Al-Awadhi, Husain Radwan, Samir |
author_sort | Khanafer, Majida |
collection | PubMed |
description | Raw, domestic sewage of Kuwait City contained about 10(6) ml(−1) colony forming units of Enterobacter hormaechei subsp. oharae (56.6%), Klebsiella spp. (36%), and Escherichia coli (7.4%), as characterized by their 16S rRNA-gene sequences. The isolated coliforms grew successfully on a mineral medium with crude oil vapor as a sole source of carbon and energy. Those strains also grew, albeit to different degrees, on individual n-alkanes with carbon chains between C(9) and C(36) and on the individual aromatic hydrocarbons, toluene, naphthalene, phenanthrene, and biphenyl as sole sources of carbon and energy. These results imply that coliforms, like other hydrocarbonoclastic microorganisms, oxidize hydrocarbons to the corresponding alcohols and then to aldehydes and fatty acids which are biodegraded by β-oxidation to acetyl CoA. The latter is a well-known key intermediate in cell material and energy production. E. coli cells grown in the presence of n-hexadecane (but not in its absence) exhibited typical intracellular hydrocarbon inclusions, as revealed by transmission electron microscopy. Raw sewage samples amended with crude oil, n-hexadecane, or phenanthrene lost these hydrocarbons gradually with time. Meanwhile, the numbers of total and individual coliforms, particularly Enterobacter, increased. It was concluded that coliform bacteria in domestic sewage, probably in other environmental materials too, are effective hydrocarbon-biodegrading microorganisms. |
format | Online Article Text |
id | pubmed-5610891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56108912017-10-29 Coliform Bacteria for Bioremediation of Waste Hydrocarbons Khanafer, Majida Al-Awadhi, Husain Radwan, Samir Biomed Res Int Research Article Raw, domestic sewage of Kuwait City contained about 10(6) ml(−1) colony forming units of Enterobacter hormaechei subsp. oharae (56.6%), Klebsiella spp. (36%), and Escherichia coli (7.4%), as characterized by their 16S rRNA-gene sequences. The isolated coliforms grew successfully on a mineral medium with crude oil vapor as a sole source of carbon and energy. Those strains also grew, albeit to different degrees, on individual n-alkanes with carbon chains between C(9) and C(36) and on the individual aromatic hydrocarbons, toluene, naphthalene, phenanthrene, and biphenyl as sole sources of carbon and energy. These results imply that coliforms, like other hydrocarbonoclastic microorganisms, oxidize hydrocarbons to the corresponding alcohols and then to aldehydes and fatty acids which are biodegraded by β-oxidation to acetyl CoA. The latter is a well-known key intermediate in cell material and energy production. E. coli cells grown in the presence of n-hexadecane (but not in its absence) exhibited typical intracellular hydrocarbon inclusions, as revealed by transmission electron microscopy. Raw sewage samples amended with crude oil, n-hexadecane, or phenanthrene lost these hydrocarbons gradually with time. Meanwhile, the numbers of total and individual coliforms, particularly Enterobacter, increased. It was concluded that coliform bacteria in domestic sewage, probably in other environmental materials too, are effective hydrocarbon-biodegrading microorganisms. Hindawi 2017 2017-09-10 /pmc/articles/PMC5610891/ /pubmed/29082238 http://dx.doi.org/10.1155/2017/1838072 Text en Copyright © 2017 Majida Khanafer et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Khanafer, Majida Al-Awadhi, Husain Radwan, Samir Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title | Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title_full | Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title_fullStr | Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title_full_unstemmed | Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title_short | Coliform Bacteria for Bioremediation of Waste Hydrocarbons |
title_sort | coliform bacteria for bioremediation of waste hydrocarbons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610891/ https://www.ncbi.nlm.nih.gov/pubmed/29082238 http://dx.doi.org/10.1155/2017/1838072 |
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