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An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences

Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter...

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Detalles Bibliográficos
Autores principales:	Moyle, Richard L., Carvalhais, Lilia C., Pretorius, Lara-Simone, Nowak, Ekaterina, Subramaniam, Gayathery, Dalton-Morgan, Jessica, Schenk, Peer M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Plant Science
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611435/ https://www.ncbi.nlm.nih.gov/pubmed/28979287 http://dx.doi.org/10.3389/fpls.2017.01631

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