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Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect

Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM an...

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Autores principales: Domínguez Rubio, A. Paula, Martínez, Jimena H., Martínez Casillas, Diana C., Coluccio Leskow, Federico, Piuri, Mariana, Pérez, Oscar E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611436/
https://www.ncbi.nlm.nih.gov/pubmed/28979244
http://dx.doi.org/10.3389/fmicb.2017.01783
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author Domínguez Rubio, A. Paula
Martínez, Jimena H.
Martínez Casillas, Diana C.
Coluccio Leskow, Federico
Piuri, Mariana
Pérez, Oscar E.
author_facet Domínguez Rubio, A. Paula
Martínez, Jimena H.
Martínez Casillas, Diana C.
Coluccio Leskow, Federico
Piuri, Mariana
Pérez, Oscar E.
author_sort Domínguez Rubio, A. Paula
collection PubMed
description Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30–50 nm). MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS), we identified a total of 103 proteins; 13 exclusively present in the MVs. The MVs content included cell envelope associated and secretory proteins, heat and cold shock proteins, several metabolic enzymes, proteases, structural components of the ribosome, membrane transporters, cell wall-associated hydrolases and phage related proteins. In particular, we identified proteins described as mediators of Lactobacillus’ probiotic effects such as p40, p75 and the product of LCABL_31160, annotated as an adhesion protein. The presence of these proteins suggests a role for the MVs in the bacteria-gastrointestinal cells interface. The expression and further encapsulation of proteins into MVs of GRAS (Generally Recognized as Safe) bacteria could represent a scientific novelty, with applications in food, nutraceuticals and clinical therapies.
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spelling pubmed-56114362017-10-04 Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect Domínguez Rubio, A. Paula Martínez, Jimena H. Martínez Casillas, Diana C. Coluccio Leskow, Federico Piuri, Mariana Pérez, Oscar E. Front Microbiol Microbiology Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30–50 nm). MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS), we identified a total of 103 proteins; 13 exclusively present in the MVs. The MVs content included cell envelope associated and secretory proteins, heat and cold shock proteins, several metabolic enzymes, proteases, structural components of the ribosome, membrane transporters, cell wall-associated hydrolases and phage related proteins. In particular, we identified proteins described as mediators of Lactobacillus’ probiotic effects such as p40, p75 and the product of LCABL_31160, annotated as an adhesion protein. The presence of these proteins suggests a role for the MVs in the bacteria-gastrointestinal cells interface. The expression and further encapsulation of proteins into MVs of GRAS (Generally Recognized as Safe) bacteria could represent a scientific novelty, with applications in food, nutraceuticals and clinical therapies. Frontiers Media S.A. 2017-09-20 /pmc/articles/PMC5611436/ /pubmed/28979244 http://dx.doi.org/10.3389/fmicb.2017.01783 Text en Copyright © 2017 Domínguez Rubio, Martínez, Martínez Casillas, Coluccio Leskow, Piuri and Pérez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Domínguez Rubio, A. Paula
Martínez, Jimena H.
Martínez Casillas, Diana C.
Coluccio Leskow, Federico
Piuri, Mariana
Pérez, Oscar E.
Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title_full Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title_fullStr Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title_full_unstemmed Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title_short Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect
title_sort lactobacillus casei bl23 produces microvesicles carrying proteins that have been associated with its probiotic effect
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611436/
https://www.ncbi.nlm.nih.gov/pubmed/28979244
http://dx.doi.org/10.3389/fmicb.2017.01783
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