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Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR

WRKY transcription factors (TFs) are involved in regulating a range of biological processes such as growth, development, and the responses to biotic and abiotic stresses. Genome-wide expression profiling of OsWRKY TF superfamily genes in rice after infection with Xanthomonas oryzae pv. oryzae (Xoo)...

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Autores principales: Choi, Nae Young, Lee, Eunhye, Lee, Sang Gu, Choi, Chang Hyun, Park, Sang Ryeol, Ahn, Ilpyung, Bae, Shin Chul, Hwang, Cheol Ho, Hwang, Duk-Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611491/
https://www.ncbi.nlm.nih.gov/pubmed/28979285
http://dx.doi.org/10.3389/fpls.2017.01628
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author Choi, Nae Young
Lee, Eunhye
Lee, Sang Gu
Choi, Chang Hyun
Park, Sang Ryeol
Ahn, Ilpyung
Bae, Shin Chul
Hwang, Cheol Ho
Hwang, Duk-Ju
author_facet Choi, Nae Young
Lee, Eunhye
Lee, Sang Gu
Choi, Chang Hyun
Park, Sang Ryeol
Ahn, Ilpyung
Bae, Shin Chul
Hwang, Cheol Ho
Hwang, Duk-Ju
author_sort Choi, Nae Young
collection PubMed
description WRKY transcription factors (TFs) are involved in regulating a range of biological processes such as growth, development, and the responses to biotic and abiotic stresses. Genome-wide expression profiling of OsWRKY TF superfamily genes in rice after infection with Xanthomonas oryzae pv. oryzae (Xoo) was performed to elucidate the function of OsWRKY TFs in the interaction between rice and Xoo. Of the 111 OsWRKY TF genes tested, the transcription of 94 genes changed after Xoo infection. The OsWRKY TF genes were classified into eight types according to their expression profiles. Eighty-two genes in Groups I, II, III, IV, VII were up-regulated after exposure to a compatible or an incompatible race of Xoo. Examination of salicylic acid (SA)-deficient rice lines revealed that SA was involved in Xa1-mediated resistance to Xoo infection. OsWRKY TF genes involved in Xa1-mediated resistance were classified according to their SA-dependent or -independent expression. In SA-deficient rice, the expression of 12 of 57 OsWRKY TF genes involved in Xa1-mediated resistance was compromised. Of these six OsWRKY TF genes were induced by SA. OsWRKY88, an example of a gene possibly involved in SA-dependent Xa1-mediated resistance, activated defense related genes and increased resistance to Xoo. Thus, expression profiling of OsWRKY TF genes may help predict the functions of OsWRKY TF genes involved in Xa1-mediated resistance.
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spelling pubmed-56114912017-10-04 Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR Choi, Nae Young Lee, Eunhye Lee, Sang Gu Choi, Chang Hyun Park, Sang Ryeol Ahn, Ilpyung Bae, Shin Chul Hwang, Cheol Ho Hwang, Duk-Ju Front Plant Sci Plant Science WRKY transcription factors (TFs) are involved in regulating a range of biological processes such as growth, development, and the responses to biotic and abiotic stresses. Genome-wide expression profiling of OsWRKY TF superfamily genes in rice after infection with Xanthomonas oryzae pv. oryzae (Xoo) was performed to elucidate the function of OsWRKY TFs in the interaction between rice and Xoo. Of the 111 OsWRKY TF genes tested, the transcription of 94 genes changed after Xoo infection. The OsWRKY TF genes were classified into eight types according to their expression profiles. Eighty-two genes in Groups I, II, III, IV, VII were up-regulated after exposure to a compatible or an incompatible race of Xoo. Examination of salicylic acid (SA)-deficient rice lines revealed that SA was involved in Xa1-mediated resistance to Xoo infection. OsWRKY TF genes involved in Xa1-mediated resistance were classified according to their SA-dependent or -independent expression. In SA-deficient rice, the expression of 12 of 57 OsWRKY TF genes involved in Xa1-mediated resistance was compromised. Of these six OsWRKY TF genes were induced by SA. OsWRKY88, an example of a gene possibly involved in SA-dependent Xa1-mediated resistance, activated defense related genes and increased resistance to Xoo. Thus, expression profiling of OsWRKY TF genes may help predict the functions of OsWRKY TF genes involved in Xa1-mediated resistance. Frontiers Media S.A. 2017-09-20 /pmc/articles/PMC5611491/ /pubmed/28979285 http://dx.doi.org/10.3389/fpls.2017.01628 Text en Copyright © 2017 Choi, Lee, Lee, Choi, Park, Ahn, Bae, Hwang and Hwang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Choi, Nae Young
Lee, Eunhye
Lee, Sang Gu
Choi, Chang Hyun
Park, Sang Ryeol
Ahn, Ilpyung
Bae, Shin Chul
Hwang, Cheol Ho
Hwang, Duk-Ju
Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title_full Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title_fullStr Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title_full_unstemmed Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title_short Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR
title_sort genome-wide expression profiling of oswrky superfamily genes during infection with xanthomonas oryzae pv. oryzae using real-time pcr
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611491/
https://www.ncbi.nlm.nih.gov/pubmed/28979285
http://dx.doi.org/10.3389/fpls.2017.01628
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