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A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola

BACKGROUND: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and b...

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Autores principales: Pigné, Sandrine, Zykwinska, Agata, Janod, Etienne, Cuenot, Stéphane, Kerkoud, Mohammed, Raulo, Roxane, Bataillé-Simoneau, Nelly, Marchi, Muriel, Kwasiborski, Anthony, N’Guyen, Guillaume, Mabilleau, Guillaume, Simoneau, Philippe, Guillemette, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611651/
https://www.ncbi.nlm.nih.gov/pubmed/28955470
http://dx.doi.org/10.1186/s40694-016-0029-3
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author Pigné, Sandrine
Zykwinska, Agata
Janod, Etienne
Cuenot, Stéphane
Kerkoud, Mohammed
Raulo, Roxane
Bataillé-Simoneau, Nelly
Marchi, Muriel
Kwasiborski, Anthony
N’Guyen, Guillaume
Mabilleau, Guillaume
Simoneau, Philippe
Guillemette, Thomas
author_facet Pigné, Sandrine
Zykwinska, Agata
Janod, Etienne
Cuenot, Stéphane
Kerkoud, Mohammed
Raulo, Roxane
Bataillé-Simoneau, Nelly
Marchi, Muriel
Kwasiborski, Anthony
N’Guyen, Guillaume
Mabilleau, Guillaume
Simoneau, Philippe
Guillemette, Thomas
author_sort Pigné, Sandrine
collection PubMed
description BACKGROUND: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. RESULTS: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Δabmak1 strain compared to the wild-type and complemented strains. We investigated the cell wall morphology and physical properties in the wild-type and transformants using electron and atomic force microscopy. These approaches confirmed the aberrant morphology of the conidial wall structure in the Δabmak1 strain which had an impact on hydrophilic adhesion and conidial surface stiffness. However, there was no significant impairment in growth, conidia formation, pathogenicity or susceptibility to various environmental stresses in the Δabmak1 strain. CONCLUSION: This study sheds new light on the function of a fungal flavin-dependent monooxygenase, which plays an important role in melanization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40694-016-0029-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-56116512017-09-27 A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola Pigné, Sandrine Zykwinska, Agata Janod, Etienne Cuenot, Stéphane Kerkoud, Mohammed Raulo, Roxane Bataillé-Simoneau, Nelly Marchi, Muriel Kwasiborski, Anthony N’Guyen, Guillaume Mabilleau, Guillaume Simoneau, Philippe Guillemette, Thomas Fungal Biol Biotechnol Research BACKGROUND: Flavin-dependent monooxygenases are involved in key biological processes as they catalyze a wide variety of chemo-, regio- and enantioselective oxygenation reactions. Flavoprotein monooxygenases are frequently encountered in micro-organisms, most of which require further functional and biocatalytic assessment. Here we investigated the function of the AbMak1 gene, which encodes a group A flavin monooxygenase in the plant pathogenic fungus Alternaria brassicicola, by generating a deficient mutant and examining its phenotype. RESULTS: Functional analysis indicates that the AbMak1 protein is involved in cell wall biogenesis and influences the melanization process. We documented a significant decrease in melanin content in the Δabmak1 strain compared to the wild-type and complemented strains. We investigated the cell wall morphology and physical properties in the wild-type and transformants using electron and atomic force microscopy. These approaches confirmed the aberrant morphology of the conidial wall structure in the Δabmak1 strain which had an impact on hydrophilic adhesion and conidial surface stiffness. However, there was no significant impairment in growth, conidia formation, pathogenicity or susceptibility to various environmental stresses in the Δabmak1 strain. CONCLUSION: This study sheds new light on the function of a fungal flavin-dependent monooxygenase, which plays an important role in melanization. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40694-016-0029-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-06 /pmc/articles/PMC5611651/ /pubmed/28955470 http://dx.doi.org/10.1186/s40694-016-0029-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Pigné, Sandrine
Zykwinska, Agata
Janod, Etienne
Cuenot, Stéphane
Kerkoud, Mohammed
Raulo, Roxane
Bataillé-Simoneau, Nelly
Marchi, Muriel
Kwasiborski, Anthony
N’Guyen, Guillaume
Mabilleau, Guillaume
Simoneau, Philippe
Guillemette, Thomas
A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title_full A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title_fullStr A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title_full_unstemmed A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title_short A flavoprotein supports cell wall properties in the necrotrophic fungus Alternaria brassicicola
title_sort flavoprotein supports cell wall properties in the necrotrophic fungus alternaria brassicicola
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611651/
https://www.ncbi.nlm.nih.gov/pubmed/28955470
http://dx.doi.org/10.1186/s40694-016-0029-3
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