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Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis

BACKGROUND AND PURPOSE: Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identification and phylogenetic marker in Candida trop...

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Autores principales: Abastabar, M, Hosseinpoor, S, Hedayati, MT, Shokohi, T, Valadan, R, Mirhendi, H, Mohammadi, R, Aghili, SR, Rahimi, N, Aslani, N, Haghani, I, Gholami, S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611690/
https://www.ncbi.nlm.nih.gov/pubmed/28959789
http://dx.doi.org/10.18869/acadpub.cmm.2.4.1
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author Abastabar, M
Hosseinpoor, S
Hedayati, MT
Shokohi, T
Valadan, R
Mirhendi, H
Mohammadi, R
Aghili, SR
Rahimi, N
Aslani, N
Haghani, I
Gholami, S
author_facet Abastabar, M
Hosseinpoor, S
Hedayati, MT
Shokohi, T
Valadan, R
Mirhendi, H
Mohammadi, R
Aghili, SR
Rahimi, N
Aslani, N
Haghani, I
Gholami, S
author_sort Abastabar, M
collection PubMed
description BACKGROUND AND PURPOSE: Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identification and phylogenetic marker in Candida tropicalis, C. orthopsilosis, C. parapsilosis and C. glabrata. MATERIALS AND METHODS: Initially, four specific primer pairs were designed, and the target was amplified and finally sequenced. A total of 77 Candida isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of nucleotide and amino acid polymorphism were assessed. RESULTS: According to the results, the specific amplified fragments of HWP1 gene were useful for the differentiation of four species. Intra-species variation was observed only in C. tropicalis with two DNA types. The phylogenetic tree of Candida species based on the HWP1 gene showed consistency in topology with those inferred from other gene sequences. CONCLUSION: We found that HWP1 gene was an excellent marker for the identification of non-albicans Candida species as well as the phylogenetic analysis of the most clinically significant Candida species.
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spelling pubmed-56116902017-09-28 Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis Abastabar, M Hosseinpoor, S Hedayati, MT Shokohi, T Valadan, R Mirhendi, H Mohammadi, R Aghili, SR Rahimi, N Aslani, N Haghani, I Gholami, S Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identification and phylogenetic marker in Candida tropicalis, C. orthopsilosis, C. parapsilosis and C. glabrata. MATERIALS AND METHODS: Initially, four specific primer pairs were designed, and the target was amplified and finally sequenced. A total of 77 Candida isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of nucleotide and amino acid polymorphism were assessed. RESULTS: According to the results, the specific amplified fragments of HWP1 gene were useful for the differentiation of four species. Intra-species variation was observed only in C. tropicalis with two DNA types. The phylogenetic tree of Candida species based on the HWP1 gene showed consistency in topology with those inferred from other gene sequences. CONCLUSION: We found that HWP1 gene was an excellent marker for the identification of non-albicans Candida species as well as the phylogenetic analysis of the most clinically significant Candida species. Iranian Society of Medical Mycology 2016-12 /pmc/articles/PMC5611690/ /pubmed/28959789 http://dx.doi.org/10.18869/acadpub.cmm.2.4.1 Text en Copyright© 2016, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International License (http://creativecommons.org/licenses/bync/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Original Article
Abastabar, M
Hosseinpoor, S
Hedayati, MT
Shokohi, T
Valadan, R
Mirhendi, H
Mohammadi, R
Aghili, SR
Rahimi, N
Aslani, N
Haghani, I
Gholami, S
Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title_full Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title_fullStr Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title_full_unstemmed Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title_short Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis
title_sort hyphal wall protein 1 gene: a potential marker for the identification of different candida species and phylogenetic analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611690/
https://www.ncbi.nlm.nih.gov/pubmed/28959789
http://dx.doi.org/10.18869/acadpub.cmm.2.4.1
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