Cargando…

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

BACKGROUND AND PURPOSE: Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternati...

Descripción completa

Detalles Bibliográficos
Autores principales: Mahmoudi, E, Saeidi, M, Marashi, MA, Moafi, A, Mahmoodi, V, Zeinolabedini Zamani, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611694/
https://www.ncbi.nlm.nih.gov/pubmed/28959793
http://dx.doi.org/10.18869/acadpub.cmm.2.4.30
_version_ 1783266000500686848
author Mahmoudi, E
Saeidi, M
Marashi, MA
Moafi, A
Mahmoodi, V
Zeinolabedini Zamani, M
author_facet Mahmoudi, E
Saeidi, M
Marashi, MA
Moafi, A
Mahmoodi, V
Zeinolabedini Zamani, M
author_sort Mahmoudi, E
collection PubMed
description BACKGROUND AND PURPOSE: Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate the antifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the patients with seborrheic dermatitis. MATERIALS AND METHODS: A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL. RESULTS: The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species, followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL. CONCLUSION: The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia.
format Online
Article
Text
id pubmed-5611694
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Iranian Society of Medical Mycology
record_format MEDLINE/PubMed
spelling pubmed-56116942017-09-28 In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis Mahmoudi, E Saeidi, M Marashi, MA Moafi, A Mahmoodi, V Zeinolabedini Zamani, M Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate the antifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the patients with seborrheic dermatitis. MATERIALS AND METHODS: A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL. RESULTS: The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species, followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL. CONCLUSION: The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia. Iranian Society of Medical Mycology 2016-12 /pmc/articles/PMC5611694/ /pubmed/28959793 http://dx.doi.org/10.18869/acadpub.cmm.2.4.30 Text en Copyright© 2016, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International License (http://creativecommons.org/licenses/bync/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Original Article
Mahmoudi, E
Saeidi, M
Marashi, MA
Moafi, A
Mahmoodi, V
Zeinolabedini Zamani, M
In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title_full In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title_fullStr In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title_full_unstemmed In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title_short In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis
title_sort in vitro activity of kombucha tea ethyl acetate fraction against malassezia species isolated from seborrhoeic dermatitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611694/
https://www.ncbi.nlm.nih.gov/pubmed/28959793
http://dx.doi.org/10.18869/acadpub.cmm.2.4.30
work_keys_str_mv AT mahmoudie invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis
AT saeidim invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis
AT marashima invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis
AT moafia invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis
AT mahmoodiv invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis
AT zeinolabedinizamanim invitroactivityofkombuchateaethylacetatefractionagainstmalasseziaspeciesisolatedfromseborrhoeicdermatitis