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Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection
Ileocecal resection (ICR), one of several types of intestinal resection that results in short bowel syndrome (SBS), causes severe clinical disease in humans. We here describe a mouse model of massive ICR in which 75% of the distal small intestine is removed. We demonstrate that mice underwent 75% IC...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612230/ https://www.ncbi.nlm.nih.gov/pubmed/28818841 http://dx.doi.org/10.1242/bio.024927 |
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author | Matsumoto, Yuka Mochizuki, Wakana Akiyama, Shintaro Matsumoto, Taichi Nozaki, Kengo Watanabe, Mamoru Nakamura, Tetsuya |
author_facet | Matsumoto, Yuka Mochizuki, Wakana Akiyama, Shintaro Matsumoto, Taichi Nozaki, Kengo Watanabe, Mamoru Nakamura, Tetsuya |
author_sort | Matsumoto, Yuka |
collection | PubMed |
description | Ileocecal resection (ICR), one of several types of intestinal resection that results in short bowel syndrome (SBS), causes severe clinical disease in humans. We here describe a mouse model of massive ICR in which 75% of the distal small intestine is removed. We demonstrate that mice underwent 75% ICR show severe clinical signs and high mortality, which may recapitulate severe forms of human SBS, despite an adaptive response throughout the remnant intestine. By using this model, we also investigated whether the epithelium of the remnant intestine shows enhanced expression of factors involved in region-specific functions of the ileum. Cubn mRNA and its protein product, which play an essential role in vitamin B12 absorption in the ileum, are not compensatory up-regulated in any part of the remnant intestine, demonstrating a clear contrast with post-operative up-regulation of genes involved in bile acid absorption. Our study suggests that functional adaptation by phenotypical changes in the intestinal epithelium is not a general feature for nutrient absorption systems that are confined to the ileum. We also propose that the mouse model developed in this study will become a unique system to facilitate studies on SBS with ICR in humans. |
format | Online Article Text |
id | pubmed-5612230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56122302017-09-29 Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection Matsumoto, Yuka Mochizuki, Wakana Akiyama, Shintaro Matsumoto, Taichi Nozaki, Kengo Watanabe, Mamoru Nakamura, Tetsuya Biol Open Research Article Ileocecal resection (ICR), one of several types of intestinal resection that results in short bowel syndrome (SBS), causes severe clinical disease in humans. We here describe a mouse model of massive ICR in which 75% of the distal small intestine is removed. We demonstrate that mice underwent 75% ICR show severe clinical signs and high mortality, which may recapitulate severe forms of human SBS, despite an adaptive response throughout the remnant intestine. By using this model, we also investigated whether the epithelium of the remnant intestine shows enhanced expression of factors involved in region-specific functions of the ileum. Cubn mRNA and its protein product, which play an essential role in vitamin B12 absorption in the ileum, are not compensatory up-regulated in any part of the remnant intestine, demonstrating a clear contrast with post-operative up-regulation of genes involved in bile acid absorption. Our study suggests that functional adaptation by phenotypical changes in the intestinal epithelium is not a general feature for nutrient absorption systems that are confined to the ileum. We also propose that the mouse model developed in this study will become a unique system to facilitate studies on SBS with ICR in humans. The Company of Biologists Ltd 2017-08-17 /pmc/articles/PMC5612230/ /pubmed/28818841 http://dx.doi.org/10.1242/bio.024927 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Matsumoto, Yuka Mochizuki, Wakana Akiyama, Shintaro Matsumoto, Taichi Nozaki, Kengo Watanabe, Mamoru Nakamura, Tetsuya Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title | Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title_full | Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title_fullStr | Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title_full_unstemmed | Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title_short | Distinct intestinal adaptation for vitamin B12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
title_sort | distinct intestinal adaptation for vitamin b12 and bile acid absorption revealed in a new mouse model of massive ileocecal resection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612230/ https://www.ncbi.nlm.nih.gov/pubmed/28818841 http://dx.doi.org/10.1242/bio.024927 |
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