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Fat-containing cells are eliminated during Dictyostelium development

Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes...

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Detalles Bibliográficos
Autores principales: Kornke, Jessica M., Maniak, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612234/
https://www.ncbi.nlm.nih.gov/pubmed/28751309
http://dx.doi.org/10.1242/bio.025478
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author Kornke, Jessica M.
Maniak, Markus
author_facet Kornke, Jessica M.
Maniak, Markus
author_sort Kornke, Jessica M.
collection PubMed
description Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes of fat metabolism, such as fcsA, encoding a fatty acid activating enzyme, or dgat1 and dgat2, specifying proteins that synthesize triacylglycerol, strongly increase their chances to contribute to the spore fraction of the developing fruiting body, but lose the ability to produce storage fat efficiently. Dictyostelium seipin, an orthologue of a human protein that in patients causes the complete loss of adipose tissue when mutated, does not quantitatively affect fat storage in the amoeba. Dictyostelium seiP knockout mutants have lipid droplets that are enlarged in size but reduced in number. These mutants are as vulnerable as the wild type when exposed to fatty acids during their vegetative growth phase, and do not efficiently enter the spore head in Dictyostelium development.
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spelling pubmed-56122342017-09-29 Fat-containing cells are eliminated during Dictyostelium development Kornke, Jessica M. Maniak, Markus Biol Open Research Article Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes of fat metabolism, such as fcsA, encoding a fatty acid activating enzyme, or dgat1 and dgat2, specifying proteins that synthesize triacylglycerol, strongly increase their chances to contribute to the spore fraction of the developing fruiting body, but lose the ability to produce storage fat efficiently. Dictyostelium seipin, an orthologue of a human protein that in patients causes the complete loss of adipose tissue when mutated, does not quantitatively affect fat storage in the amoeba. Dictyostelium seiP knockout mutants have lipid droplets that are enlarged in size but reduced in number. These mutants are as vulnerable as the wild type when exposed to fatty acids during their vegetative growth phase, and do not efficiently enter the spore head in Dictyostelium development. The Company of Biologists Ltd 2017-07-27 /pmc/articles/PMC5612234/ /pubmed/28751309 http://dx.doi.org/10.1242/bio.025478 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Kornke, Jessica M.
Maniak, Markus
Fat-containing cells are eliminated during Dictyostelium development
title Fat-containing cells are eliminated during Dictyostelium development
title_full Fat-containing cells are eliminated during Dictyostelium development
title_fullStr Fat-containing cells are eliminated during Dictyostelium development
title_full_unstemmed Fat-containing cells are eliminated during Dictyostelium development
title_short Fat-containing cells are eliminated during Dictyostelium development
title_sort fat-containing cells are eliminated during dictyostelium development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612234/
https://www.ncbi.nlm.nih.gov/pubmed/28751309
http://dx.doi.org/10.1242/bio.025478
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