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Fat-containing cells are eliminated during Dictyostelium development
Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612234/ https://www.ncbi.nlm.nih.gov/pubmed/28751309 http://dx.doi.org/10.1242/bio.025478 |
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author | Kornke, Jessica M. Maniak, Markus |
author_facet | Kornke, Jessica M. Maniak, Markus |
author_sort | Kornke, Jessica M. |
collection | PubMed |
description | Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes of fat metabolism, such as fcsA, encoding a fatty acid activating enzyme, or dgat1 and dgat2, specifying proteins that synthesize triacylglycerol, strongly increase their chances to contribute to the spore fraction of the developing fruiting body, but lose the ability to produce storage fat efficiently. Dictyostelium seipin, an orthologue of a human protein that in patients causes the complete loss of adipose tissue when mutated, does not quantitatively affect fat storage in the amoeba. Dictyostelium seiP knockout mutants have lipid droplets that are enlarged in size but reduced in number. These mutants are as vulnerable as the wild type when exposed to fatty acids during their vegetative growth phase, and do not efficiently enter the spore head in Dictyostelium development. |
format | Online Article Text |
id | pubmed-5612234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-56122342017-09-29 Fat-containing cells are eliminated during Dictyostelium development Kornke, Jessica M. Maniak, Markus Biol Open Research Article Triacylglycerol is a universal storage molecule for metabolic energy in living organisms. However, Dictyostelium amoebae, that have accumulated storage fat from added fatty acids do not progress through the starvation period preceding the development of the durable spore. Mutants deficient in genes of fat metabolism, such as fcsA, encoding a fatty acid activating enzyme, or dgat1 and dgat2, specifying proteins that synthesize triacylglycerol, strongly increase their chances to contribute to the spore fraction of the developing fruiting body, but lose the ability to produce storage fat efficiently. Dictyostelium seipin, an orthologue of a human protein that in patients causes the complete loss of adipose tissue when mutated, does not quantitatively affect fat storage in the amoeba. Dictyostelium seiP knockout mutants have lipid droplets that are enlarged in size but reduced in number. These mutants are as vulnerable as the wild type when exposed to fatty acids during their vegetative growth phase, and do not efficiently enter the spore head in Dictyostelium development. The Company of Biologists Ltd 2017-07-27 /pmc/articles/PMC5612234/ /pubmed/28751309 http://dx.doi.org/10.1242/bio.025478 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Kornke, Jessica M. Maniak, Markus Fat-containing cells are eliminated during Dictyostelium development |
title | Fat-containing cells are eliminated during Dictyostelium development |
title_full | Fat-containing cells are eliminated during Dictyostelium development |
title_fullStr | Fat-containing cells are eliminated during Dictyostelium development |
title_full_unstemmed | Fat-containing cells are eliminated during Dictyostelium development |
title_short | Fat-containing cells are eliminated during Dictyostelium development |
title_sort | fat-containing cells are eliminated during dictyostelium development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612234/ https://www.ncbi.nlm.nih.gov/pubmed/28751309 http://dx.doi.org/10.1242/bio.025478 |
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