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Dietary phosphate exacerbates intestinal inflammation in experimental colitis

The recent widespread consumption of Western diets and food additives worldwide is associated with excessive inorganic phosphate intake. However, researchers have known little about the impact of dietary phosphate intake on the development of inflammatory bowel disease to date. In this study, we inv...

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Autores principales: Sugihara, Kohei, Masuda, Masashi, Nakao, Mari, Abuduli, Maerjianghan, Imi, Yukiko, Oda, Naoko, Okahisa, Toshiya, Yamamoto, Hironori, Takeda, Eiji, Taketani, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612814/
https://www.ncbi.nlm.nih.gov/pubmed/28955125
http://dx.doi.org/10.3164/jcbn.16-117
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author Sugihara, Kohei
Masuda, Masashi
Nakao, Mari
Abuduli, Maerjianghan
Imi, Yukiko
Oda, Naoko
Okahisa, Toshiya
Yamamoto, Hironori
Takeda, Eiji
Taketani, Yutaka
author_facet Sugihara, Kohei
Masuda, Masashi
Nakao, Mari
Abuduli, Maerjianghan
Imi, Yukiko
Oda, Naoko
Okahisa, Toshiya
Yamamoto, Hironori
Takeda, Eiji
Taketani, Yutaka
author_sort Sugihara, Kohei
collection PubMed
description The recent widespread consumption of Western diets and food additives worldwide is associated with excessive inorganic phosphate intake. However, researchers have known little about the impact of dietary phosphate intake on the development of inflammatory bowel disease to date. In this study, we investigated the effects of dietary phosphate on intestinal inflammation in experimental colitis. Sprague-Dawley rats were fed different phosphate diets (0.5%, 1.0% and 1.5% phosphate) with or without dextran sulfate sodium. For in vitro study, the effects of phosphate on proinflammatory cytokine induction and reactive oxygen species production in RAW264.7 macrophage were examined. Dietary phosphate exacerbated intestinal inflammation in experimental colitis in a dose-dependent manner, as assessed by the clinical disease activity score, colon length, and histology. Furthermore, the high phosphate diet increased myeloperoxidase activity and proinflammatory cytokine mRNA expression through the activation of nuclear factor κB in the inflamed colon. In addition, high phosphate loading in RAW264.7 cells directly enhanced reactive oxygen species production and proinflammatory cytokine gene expression. Our results demonstrated that the high phosphate diet exacerbated intestinal inflammation in experimental colitis. These findings have important therapeutic implications for inflammatory bowel disease patients.
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spelling pubmed-56128142017-09-27 Dietary phosphate exacerbates intestinal inflammation in experimental colitis Sugihara, Kohei Masuda, Masashi Nakao, Mari Abuduli, Maerjianghan Imi, Yukiko Oda, Naoko Okahisa, Toshiya Yamamoto, Hironori Takeda, Eiji Taketani, Yutaka J Clin Biochem Nutr Original Article The recent widespread consumption of Western diets and food additives worldwide is associated with excessive inorganic phosphate intake. However, researchers have known little about the impact of dietary phosphate intake on the development of inflammatory bowel disease to date. In this study, we investigated the effects of dietary phosphate on intestinal inflammation in experimental colitis. Sprague-Dawley rats were fed different phosphate diets (0.5%, 1.0% and 1.5% phosphate) with or without dextran sulfate sodium. For in vitro study, the effects of phosphate on proinflammatory cytokine induction and reactive oxygen species production in RAW264.7 macrophage were examined. Dietary phosphate exacerbated intestinal inflammation in experimental colitis in a dose-dependent manner, as assessed by the clinical disease activity score, colon length, and histology. Furthermore, the high phosphate diet increased myeloperoxidase activity and proinflammatory cytokine mRNA expression through the activation of nuclear factor κB in the inflamed colon. In addition, high phosphate loading in RAW264.7 cells directly enhanced reactive oxygen species production and proinflammatory cytokine gene expression. Our results demonstrated that the high phosphate diet exacerbated intestinal inflammation in experimental colitis. These findings have important therapeutic implications for inflammatory bowel disease patients. the Society for Free Radical Research Japan 2017-09 2017-07-28 /pmc/articles/PMC5612814/ /pubmed/28955125 http://dx.doi.org/10.3164/jcbn.16-117 Text en Copyright © 2017 JCBN http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sugihara, Kohei
Masuda, Masashi
Nakao, Mari
Abuduli, Maerjianghan
Imi, Yukiko
Oda, Naoko
Okahisa, Toshiya
Yamamoto, Hironori
Takeda, Eiji
Taketani, Yutaka
Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title_full Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title_fullStr Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title_full_unstemmed Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title_short Dietary phosphate exacerbates intestinal inflammation in experimental colitis
title_sort dietary phosphate exacerbates intestinal inflammation in experimental colitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612814/
https://www.ncbi.nlm.nih.gov/pubmed/28955125
http://dx.doi.org/10.3164/jcbn.16-117
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