Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells

The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration...

Descripción completa

Detalles Bibliográficos
Autores principales: Góral, Małgorzata, Pankiewicz, Urszula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613034/
https://www.ncbi.nlm.nih.gov/pubmed/28871398
http://dx.doi.org/10.1007/s00232-017-9986-6
_version_ 1783266168894652416
author Góral, Małgorzata
Pankiewicz, Urszula
author_facet Góral, Małgorzata
Pankiewicz, Urszula
author_sort Góral, Małgorzata
collection PubMed
description The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg(2+)/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg(2+) may be an alternative for pharmacological supplementation applied in deficiency of this cation.
format Online
Article
Text
id pubmed-5613034
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-56130342017-10-10 Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells Góral, Małgorzata Pankiewicz, Urszula J Membr Biol Article The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg(2+)/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg(2+) may be an alternative for pharmacological supplementation applied in deficiency of this cation. Springer US 2017-09-04 2017 /pmc/articles/PMC5613034/ /pubmed/28871398 http://dx.doi.org/10.1007/s00232-017-9986-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Góral, Małgorzata
Pankiewicz, Urszula
Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title_full Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title_fullStr Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title_full_unstemmed Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title_short Effect of Pulsed Electric Fields (PEF) on Accumulation of Magnesium in Lactobacillus rhamnosus B 442 Cells
title_sort effect of pulsed electric fields (pef) on accumulation of magnesium in lactobacillus rhamnosus b 442 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613034/
https://www.ncbi.nlm.nih.gov/pubmed/28871398
http://dx.doi.org/10.1007/s00232-017-9986-6
work_keys_str_mv AT goralmałgorzata effectofpulsedelectricfieldspefonaccumulationofmagnesiuminlactobacillusrhamnosusb442cells
AT pankiewiczurszula effectofpulsedelectricfieldspefonaccumulationofmagnesiuminlactobacillusrhamnosusb442cells

Ejemplares similares