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Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans

Obesity leads to an altered adipocytokine production negatively effecting the function of natural killer cells (NK cells), which are important effector cells of the innate immune system. NK cells provide a defence against tumour cells or virus infected cells and have different activating and inhibit...

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Autores principales: Rosenstock, Philip, Horstkorte, Rüdiger, Gnanapragassam, Vinayaga Srinivasan, Harth, Jörg, Kielstein, Heike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613057/
https://www.ncbi.nlm.nih.gov/pubmed/28786023
http://dx.doi.org/10.1007/s12026-017-8942-y
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author Rosenstock, Philip
Horstkorte, Rüdiger
Gnanapragassam, Vinayaga Srinivasan
Harth, Jörg
Kielstein, Heike
author_facet Rosenstock, Philip
Horstkorte, Rüdiger
Gnanapragassam, Vinayaga Srinivasan
Harth, Jörg
Kielstein, Heike
author_sort Rosenstock, Philip
collection PubMed
description Obesity leads to an altered adipocytokine production negatively effecting the function of natural killer cells (NK cells), which are important effector cells of the innate immune system. NK cells provide a defence against tumour cells or virus infected cells and have different activating and inhibitory surface receptors to distinguish between normal and transformed cells. One group of the inhibitory receptors are the sialic acid-binding immunoglobulin-like lectins (Siglecs). The aim of this study was to compare the expression of Siglecs-7, -9 and -10 on NK cells from normal weight and obese subjects. Therefore peripheral blood mononuclear cells (PBMC) were isolated from 10 normal weight (BMI < 25 kg/m(2)) and 11 obese (BMI > 30 kg/m(2)) blood donors and analysed by flow cytometry. Moreover, the amount of sialic acid on NK cell was determined using a fluorescent labelled lectin that binds terminal sialic acids. Percentages of immune cells were not altered between normal weight and obese individuals. CD56(bright) NK cells from obese subjects had a reduced expression of Siglec-7 while the expression of Siglec-9 was not altered. The reduction of Siglec-7 expression on CD56(bright) NK cells might be a marker for their dysfunction. Moreover, Siglecs-7, -9 and -10 are not expressed on the NK cell lines NK-92 and NKL. When comparing the two NK cell subpopulations CD56(bright) and CD56(dim), CD56(bright) NK cells had a higher amount of sialic acids on their surface compared to CD56(dim) NK cells regardless of body weight.
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spelling pubmed-56130572017-10-10 Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans Rosenstock, Philip Horstkorte, Rüdiger Gnanapragassam, Vinayaga Srinivasan Harth, Jörg Kielstein, Heike Immunol Res Original Article Obesity leads to an altered adipocytokine production negatively effecting the function of natural killer cells (NK cells), which are important effector cells of the innate immune system. NK cells provide a defence against tumour cells or virus infected cells and have different activating and inhibitory surface receptors to distinguish between normal and transformed cells. One group of the inhibitory receptors are the sialic acid-binding immunoglobulin-like lectins (Siglecs). The aim of this study was to compare the expression of Siglecs-7, -9 and -10 on NK cells from normal weight and obese subjects. Therefore peripheral blood mononuclear cells (PBMC) were isolated from 10 normal weight (BMI < 25 kg/m(2)) and 11 obese (BMI > 30 kg/m(2)) blood donors and analysed by flow cytometry. Moreover, the amount of sialic acid on NK cell was determined using a fluorescent labelled lectin that binds terminal sialic acids. Percentages of immune cells were not altered between normal weight and obese individuals. CD56(bright) NK cells from obese subjects had a reduced expression of Siglec-7 while the expression of Siglec-9 was not altered. The reduction of Siglec-7 expression on CD56(bright) NK cells might be a marker for their dysfunction. Moreover, Siglecs-7, -9 and -10 are not expressed on the NK cell lines NK-92 and NKL. When comparing the two NK cell subpopulations CD56(bright) and CD56(dim), CD56(bright) NK cells had a higher amount of sialic acids on their surface compared to CD56(dim) NK cells regardless of body weight. Springer US 2017-08-07 2017 /pmc/articles/PMC5613057/ /pubmed/28786023 http://dx.doi.org/10.1007/s12026-017-8942-y Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Rosenstock, Philip
Horstkorte, Rüdiger
Gnanapragassam, Vinayaga Srinivasan
Harth, Jörg
Kielstein, Heike
Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title_full Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title_fullStr Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title_full_unstemmed Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title_short Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans
title_sort siglec-7 expression is reduced on a natural killer (nk) cell subset of obese humans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613057/
https://www.ncbi.nlm.nih.gov/pubmed/28786023
http://dx.doi.org/10.1007/s12026-017-8942-y
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