Cargando…

An appropriate loading control for western blot analysis in animal models of myocardial ischemic infarction

An appropriate loading control is critical for Western blot analysis. Housekeeping proteins (HKPs), such as β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin, are commonly used to normalize protein expression. But HKP expression can be impacted by certain experimental conditio...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nie, Xin, Li, Chen, Hu, Sheng, Xue, Fulai, Kang, Y. James, Zhang, Wenjing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5613232/ https://www.ncbi.nlm.nih.gov/pubmed/28955798 http://dx.doi.org/10.1016/j.bbrep.2017.09.001

Ejemplares similares

Single-cell western blotting
por: Hughes, Alex J., et al.
Publicado: (2014)

BlotIt—Optimal alignment of Western blot and qPCR experiments
por: Kemmer, Svenja, et al.
Publicado: (2022)

Comprehensive Optimization of Western Blotting
por: Liu, Dishiwen, et al.
Publicado: (2023)

HTLV-I proviral load in Argentinean subjects with indeterminate western blot patterns
por: Mangano, Andrea, et al.
Publicado: (2011)

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)
por: Gilda, Jennifer E., et al.
Publicado: (2015)

Improving rigor and reproducibility in western blot experiments with the blotRig analysis software
por: Omondi, Cleopa, et al.
Publicado: (2023)

Western Blot: Protein Transfer Overview
por: Gibbons, Janay
Publicado: (2014)

Subcellular western blotting of single cells
por: Yamauchi, Kevin A., et al.
Publicado: (2017)

Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence
por: Franke, Maximilian, et al.
Publicado: (2021)

Total Protein Analysis as a Reliable Loading Control for Quantitative Fluorescent Western Blotting
por: Eaton, Samantha L., et al.
Publicado: (2013)

Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them
por: Kroon, Cristina, et al.
Publicado: (2022)

Western Blot: Technique, Theory, and Trouble Shooting
por: Mahmood, Tahrin, et al.
Publicado: (2012)

The Design of a Quantitative Western Blot Experiment
por: Taylor, Sean C., et al.
Publicado: (2014)

Western Blot: Technique, Theory and Trouble Shooting
por: Liu, Zhi-Qiang, et al.
Publicado: (2014)

Quantifying western blots: none more black
por: Bell, Graham
Publicado: (2016)

A fixation method for the optimisation of western blotting
por: Xu, Jing, et al.
Publicado: (2019)

Antibody validation for Western blot: By the user, for the user
por: Pillai-Kastoori, Lakshmi, et al.
Publicado: (2020)

Comparison of Automated and Traditional Western Blotting Methods
por: Sormunen, Aino, et al.
Publicado: (2023)

Enhancing single-cell western blotting sensitivity using diffusive analyte blotting and antibody conjugate amplification
por: Long, Mariia Alibekova, et al.
Publicado: (2023)

Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array
por: Koch, Rick J., et al.
Publicado: (2018)

Use of a Western blot technique for the serodiagnosis of glanders
por: Elschner, Mandy C, et al.
Publicado: (2011)

Identification and validation of rice reference proteins for western blotting
por: Li, Xiaoming, et al.
Publicado: (2011)

Utility of Western Blot Analysis for the Diagnosis of Cutaneous Leishmaniasis
por: ASHRAFMANSOURI, Marzieh, et al.
Publicado: (2015)

Microfluidic Western Blotting of Low-Molecular-Mass Proteins
por: Gerver, Rachel E., et al.
Publicado: (2014)

Sensitive detection of fluorescence in western blotting by merging images
por: Kondo, Yukari, et al.
Publicado: (2018)

Phenylglyoxal-Based Visualization of Citrullinated Proteins on Western Blots
por: Hensen, Sanne M. M., et al.
Publicado: (2015)

Differentiation of endogenous erythropoietin and exogenous ESAs by Western blotting
por: Yasuoka, Yukiko, et al.
Publicado: (2020)

Aptamer-Based Western Blot for Selective Protein Recognition
por: Wang, Yao, et al.
Publicado: (2020)

Feline calicivirus protein synthesis investigated by Western blotting
por: Carter, M. J.
Publicado: (1989)

Measuring protein levels in planarians using western blotting
por: Ziman, Benjamin, et al.
Publicado: (2021)

Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
por: Zang, Jingjing, et al.
Publicado: (2023)

A theoretical timeline for myocardial infarction: immunohistochemical evaluation and western blot quantification for Interleukin-15 and Monocyte chemotactic protein-1 as very early markers
por: Turillazzi, Emanuela, et al.
Publicado: (2014)

Evaluating Strategies to Normalise Biological Replicates of Western Blot Data
por: Degasperi, Andrea, et al.
Publicado: (2014)

Validation of western blot for Histoplasma capsulatum antibody detection assay
por: Almeida, Marcos de Abreu, et al.
Publicado: (2016)

A Defined Methodology for Reliable Quantification of Western Blot Data
por: Taylor, Sean C., et al.
Publicado: (2013)

Antigen retrieval by citrate solution improves western blot signal
por: Patiño-García, Daniel, et al.
Publicado: (2019)

Comparisons of ELISA and Western blot assays for detection of autophagy flux
por: Oh, Sung-hee, et al.
Publicado: (2017)

Optimization of western blotting for the detection of proteins of different molecular weight
por: Heda, Ghanshyam D, et al.
Publicado: (2020)

Evaluation of antibodies for western blot analysis of frataxin protein isoforms
por: Weng, Liwei, et al.
Publicado: (2019)

Comparison of the sensitivity of Western blotting between PVDF and NC membranes
por: Xiang, Yufang, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_8nacdh2s3sb11enkoa9rsqb2v7